Isolation and characterization of Df(2R)BSC821
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC821 was isolated as a FLP recombinase-induced recombination event involving PBac{RB}CG30387e00881 and P{XP}Fkbp13d02985. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker markers in progeny of P{hsFLP}1, y1 w1118; PBac{RB}CG30387e00881/P{XP}Fkbp13d02985 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.RB5}BSC821 from the segment of PBac{RB}CG30387e00881 to the left of its FRT site and the segment of P{XP}Fkbp13d02985 to the right of its FRT site. The breakpoints of Df(2R)BSC821 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2R:17189303 ;17384714 and the cytological breakpoints predicted from these coordinates are 57D10;57E6. Df(2R)BSC821 failed to complement dom3 and dom9.