Isolation and characterization of Df(1)BSC843
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC843 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}cinf05298 and P{XP}d11753. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of PBac{WH}cinf05298/P{XP}d11753; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC843 from the segment of PBac{WH}cinf05298 to the left of its FRT site and the segment of P{XP}d11753 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d11753 to be Release 3 genomic coordinate 92123 on the X chromosome, which corresponds to Release 5 coordinate  X:228718 . The insertion site of PBac{WH}cinf05298 is Release 5 genomic coordinate 149001..149310 on the X chromosome. Consequently, the breakpoints of Df(1)BSC843 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  X:149001..149310 ;228718 and the cytological breakpoints predicted from these coordinates are 1A1;1A3. It failed to complement ewg2 and was rescued by Dp(1;Y)y261l.