FlyBase Allele Report: Dmel\ewg[2]

General Information

Symbol

Dmel\ewg²

Species

D. melanogaster

Name

FlyBase ID

FBal0003888

Feature type

allele

Associated gene

Dmel\ewg

Associated Insertion(s)

Carried in Construct

Also Known As

ewg^l1

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(DeSimone et al., 1996)

Mutagen

ethyl methanesulfonate

(DeSimone et al., 1996, de la Pompa et al., 1989)

Progenitor genotype

Cytology

Description

C to T base pair change in exon B resulting in the termination of the ORF at amino acid 187. The DNA binding domain is deleted. Exon B is part of all putative ewg protein isoforms.

(Koushika et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:273,859..273,859 [-]

Nucleotide change:

C273859T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

Q187term

(Koushika et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | somatic clone

(Haussmann et al., 2008)

abnormal neuroanatomy, with ewg^ΔDJ.elav

(Haussmann and Soller, 2010)

abnormal neuroanatomy | larval stage (with ewg^G1518)

(Haussmann et al., 2008)

abnormal neuroanatomy | somatic clone | third instar larval stage

(Haussmann et al., 2008)

lethal

(de la Pompa et al., 1989)

lethal | recessive

(de Simone and White, 1993)

lethal - all die during embryonic stage

(Xin et al., 2011, Haussmann and Soller, 2010)

lethal - all die during embryonic stage (with ewg¹)

(DeSimone et al., 1996)

lethal - all die during embryonic stage | recessive

(Haussmann et al., 2008, Fleming et al., 1989)

some die during embryonic stage

(Xin et al., 2011)

some die during embryonic stage (with ewg¹)

(DeSimone et al., 1996)

some die during embryonic stage | recessive

(Haussmann et al., 2008, Fleming et al., 1989)

viable, with ewg^SC3ORF.elav

(Xin et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adult ewg¹/ewg² transheterozygotes do not show any significant increase in the number of progenitor cells (intestinal stem cells and enteroblasts) in the midgut, as compared to controls.

(Tian et al., 2017)

Generation of ewg² mutant clones in the retina does not result in either defects in the specification of photoreceptors in the dorsal rim area, or photoreceptor death.

No defects in RP2 motor neuron specification or migration are seen in ewg² mutant embryos. Epithelial cell fate specification also appears normal.

Flies expressing ewg^elav.NS in a ewg² mutant background are viable but display loss of indirect flight muscles. The usual six dorsal longitudinal (DLMs) and seven dorsal ventral muscles (DVMs) on each side of the midline are all lost to be replaced by two large misshapen muscles.

No indirect flight muscle loss is seen in heterozygous ewg².

(Xin et al., 2011)

Late stage ewg² mutant embryos are fully developed and morphologically normal, but fail to hatch.

(Haussmann and Soller, 2010)

Homozygous ewg² mutant fat body clones do not exhibit any mitochondrial phenotypes.

(Baltzer et al., 2009)

Larvae carrying ewg² clones (which have been obtained by using FLP/FRT mediated recombination to inactivate the ewg^elav.PH rescuing transgene in a ewg² background in late embryogenesis) move indistinguishably from their control siblings, pupate normally and many adults hatch. The adults are impaired in walking.

At third instar neuromuscular junctions, ewg² motorneurons (which have been obtained by using FLP/FRT mediated recombination to inactivate the ewg^elav.PH rescuing transgene in a ewg² background in late embryogenesis) have an increased number of synaptic boutons (there is an 85% increase in the number of type 1b boutons at muscle 13).

There is an increase in bouton number at the larval neuromuscular junction in ewg^G1518/ewg² animals compared to wild type.

(Haussmann et al., 2008)

ewg¹/ewg² pupae exhibit severe IFM defects: a complete loss or severe reduction in the width of the affected muscles.

(DeSimone et al., 1996)

late embryonic lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ewg² has abnormal neuroanatomy | larval stage phenotype, suppressible by Hsap\NRF1^elav.PH

(Haussmann et al., 2008)

ewg² has lethal | recessive | embryonic stage phenotype, suppressible by Hsap\NRF1^elav.PH

(Haussmann et al., 2008)

NOT suppressed by

Statement

Reference

ewg² has lethal - all die during embryonic stage phenotype, non-suppressible by Hsap\NRF1^elav.PH

(Haussmann and Soller, 2010)

ewg² has abnormal neuroanatomy | somatic clone | third instar larval stage phenotype, non-suppressible by Ctet\tetX^TNT-LC.UAS/Scer\GAL4^elav.PH

(Haussmann et al., 2008)

Suppressor of

Statement

Reference

ewg¹/ewg² is a suppressor of abnormal size | adult stage phenotype of Apc^Q8

(Xin et al., 2011)

ewg^P1/ewg² is a suppressor of abnormal size | adult stage phenotype of Apc^Q8

(Xin et al., 2011)

ewg¹/ewg² is a suppressor of cell lethal | adult stage phenotype of Apc^Q8

(Xin et al., 2011)

ewg^P1/ewg² is a suppressor of cell lethal | adult stage phenotype of Apc^Q8

(Xin et al., 2011)

Other

Statement

Reference

Hsap\NRF1^elav.PH, ewg² has lethal | pharate adult stage phenotype

(Haussmann et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

ewg² has NMJ bouton phenotype, suppressible by Hsap\NRF1^elav.PH

(Haussmann et al., 2008)

ewg² has embryonic/larval neuromuscular junction phenotype, suppressible by Hsap\NRF1^elav.PH

(Haussmann et al., 2008)

NOT suppressed by

Statement

Reference

ewg² has NMJ bouton | somatic clone phenotype, non-suppressible by Ctet\tetX^TNT-LC.UAS/Scer\GAL4^elav.PH

(Haussmann et al., 2008)

ewg² has embryonic/larval neuromuscular junction | somatic clone phenotype, non-suppressible by Ctet\tetX^TNT-LC.UAS/Scer\GAL4^elav.PH

(Haussmann et al., 2008)

Enhancer of

Statement

Reference

ewg[+]/ewg² is an enhancer of indirect flight muscle cell phenotype of Scer\GAL4^ebd1.PB, pan^ΔN.UAS

(Xin et al., 2011)

ewg[+]/ewg² is an enhancer of indirect flight muscle cell phenotype of ebd1²⁴⁰

(Xin et al., 2011)

Suppressor of

Statement

Reference

ewg¹/ewg² is a suppressor of photoreceptor | adult stage phenotype of Apc^Q8

(Xin et al., 2011)

ewg^P1/ewg² is a suppressor of photoreceptor | adult stage phenotype of Apc^Q8

(Xin et al., 2011)

ewg¹/ewg² is a suppressor of rhabdomere | adult stage phenotype of Apc^Q8

(Xin et al., 2011)

ewg^P1/ewg² is a suppressor of rhabdomere | adult stage phenotype of Apc^Q8

(Xin et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Hemizygous ewg¹/ewg² suppresses the apoptosis seen in Apc^Q8 mutant photoreceptors. The shortening of photoreceptor length seen prior to apoptosis is also suppressed, as is the rhabdomere enlargement seen as a result of misspecification of all of the photoreceptors in the dorsal half of the retina to a dorsal rim area fate.

Hemizygous ewg^P1/ewg² suppresses the apoptosis seen in Apc^Q8 mutant photoreceptors. The shortening of photoreceptor length seen prior to apoptosis is also suppressed, and the rhabdomere enlargement seen as a result of misspecification of all of the photoreceptors in the dorsal half of the retina to a dorsal rim area fate is also partially suppressed. This is most evident near the dorsal-ventral equator.

One copy of ewg² enhances the indirect flight muscle defects seen when pan^ΔN.Scer\UAS is expressed under the control of Scer\GAL4^ebd1.PB.

One copy of ewg² enhances the indirect flight muscle loss seen in ebd1²⁴⁰ mutant flies.

(Xin et al., 2011)

Expression of either N^{dsRNA.P.Scer\UAS}, N^Scer\UAS.cMa or tkv^{Q199D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PH in ewg² motorneurons (these motorneurons have been obtained by using FLP/FRT mediated recombination to inactivate the ewg^elav.PH rescuing transgene and simultaneously activate the Scer\GAL4^elav.PH driver in a ewg² background) results in intermediate numbers of boutons at the larval neuromuscular junction compared to wild-type and ewg² motorneurons.

(Haussmann et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of Ctet\TeTxLC^TNT.Scer\UAS under the control of Scer\GAL4^elav.PH has no effect on the increase in bouton number seen at the neuromuscular junction of third larval instar ewg² motorneurons (these motorneurons have been obtained by using FLP/FRT mediated recombination to inactivate the ewg^elav.PH rescuing transgene and simultaneously activate the Scer\GAL4^elav.PH driver in a ewg² background in late embryogenesis).

The increase in bouton number seen at the larval neuromuscular junctions of ewg² motorneurons is completely rescued by Hsap\NRF1^elav.PH.

ewg² animals expressing Hsap\NRF1^elav.PH develop to pharate adults, but fail to hatch.

(Haussmann et al., 2008)

Complementation and Rescue Data

Rescued by

ewg² is rescued by ewg::Dvir\ewg^{tcgERΔ7v.elav.Tag:HA,Tag:VSV-G}

(Haussmann et al., 2011)

ewg² is rescued by ewg^{elav.Tag:HA,Tag:VSV-G}

(Haussmann and Soller, 2010)

ewg² is rescued by ewg^ΔD.elav

(Haussmann and Soller, 2010)

ewg² is rescued by ewg^ΔJ.elav

(Haussmann and Soller, 2010)

ewg² is rescued by ewg^elav.PH

(Haussmann et al., 2008)

ewg² is rescued by ewg^hs.PD

(de Simone and White, 1993)

ewg² is rescued by ewg^+t11.5

(Fleming et al., 1989)

ewg² is rescued by ewg^+t9.5

(Fleming et al., 1989)

Partially rescued by

ewg² is partially rescued by ewg^ΔDJ.elav

(Haussmann and Soller, 2010)

ewg¹/ewg² is partially rescued by ewg^SC3ORF.elav

(DeSimone et al., 1996)

Not rescued by

ewg² is not rescued by ewg^HH

(Fleming et al., 1989)

ewg² is not rescued by ewg^EB

(Fleming et al., 1989)

Comments

ewg::Dvir\ewg^{tcgERΔ7v.elav.T:Ivir\HA1,T:VSV\G} rescues the lethality of ewg² mutants.

(Haussmann et al., 2011)

Expression of ewg^elav.NS rescues the embryonic lethality seen in ewg² mutants.

(Xin et al., 2011)

Expression of ewg^SC3ORF.elav rescues the viability of ewg² mutants.

(Haussmann and Soller, 2010)

The increase in bouton number seen at the larval neuromuscular junctions of ewg² motorneurons is partially rescued by ewg^elav.NS.

(Haussmann et al., 2008)

ewg¹/ewg² lethality can be rescued by ewg^elav.NS. Rescued adults exhibit an erect wing phenotype due to complete absence of DLMs with varying degree of DVM defects. Introduction of ewg^hs.PD to ewg² ewg^elav.NS flies restores DVM and DLM muscles so the adults can fly.

(DeSimone et al., 1996)

ewg^hs.PD rescues lethality associated with ewg².

(de Simone and White, 1993)

Lethal phenotype can be rescued by insertions of ewg^+t11.5 and ewg^+t9.5, but cannot be rescued by insertions of ewg^EB or ewg^HH. The lethal phenotype is completely rescued by a P element carrying wild type ewg gene copy inserted into an autosomal chromosome, but only partially rescued by an insertion into the X chromosome. Rescue is not achieved by fragments of the ewg gene region.

(Fleming et al., 1989)

Images (0)

Mutant

Wild-type

Stocks (6)

Bloomington

39613

ewg² y¹ w¹ sn³; P{ewg^HS1}3

77898

y¹ w^* sn^* ewg²; P{elav-ewg.ΔDJ}DJ21

38406

ewg² y¹ w¹ sn³/FM6/Dp(1;Y)y²61l

4750

ewg² y¹ cho^* sn^*/FM6, l(1)FMa¹/Dp(1;Y)y²61l

77893

y¹ w^* ewg² P{elav(FRT.ewg)GAL4.eFeG}1/FM7h; P{UAS-mCD8::GFP.L}LL5/CyO, P{ActGFP}JMR1

Kyoto

107858

ewg² y¹ cho^* sn^*/FM6, l(1)FMa¹/Dp(1;Y)y²61l

Notes on Origin

Discoverer

White.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ewg²

(Walther et al., 2020, Tian et al., 2017, Xin et al., 2011, Popodi et al., 2010-, Baltzer et al., 2009, Christensen et al., 2009.7.21)

ewg^l1

(Haussmann et al., 2011, Haussmann and Soller, 2010, Haussmann et al., 2008, Koushika et al., 2000, Koushika et al., 1999, DeSimone et al., 1996, de Simone and White, 1993, de la Pompa et al., 1989, Fleming et al., 1989)

Name Synonyms

Secondary FlyBase IDs

References (16)

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