FlyBase Allele Report: Dmel\ebd1[240]

General Information

Symbol

Dmel\ebd1²⁴⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0258660

Feature type

allele

Associated gene

Dmel\ebd1

Associated Insertion(s)

P{?EPgy2}ebd1²⁴⁰

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Benchabane et al., 2011)

Progenitor genotype

P{EPgy2}ebd1^EY01876

(Benchabane et al., 2011)

Associated Insertion(s)

P{?EPgy2}ebd1²⁴⁰

Cytology

Description

Imprecise excision of the progenitor insertion has resulted in a 2925bp deletion that extends from +308 to +3232 relative to the ebd1 transcription start site. Approximately 3kb of the original insertion remains.

(Benchabane et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3L:682,313..685,237 [-]

Comment:

2925 bp deletion resulting from the imprecise excision of P{EPgy2}ebd1^EY01876. Extends from +308 to +3232 relative to the ebd1 transcription start site. 3L:685545 (R5) was used as the transcription start site for the mapping. Approximately 3kb of the original insertion remains.

(Benchabane et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

The adult midgut defects (excess of mitotic cells, aberrant epithelium architecture) characteristic for Apc[Q8] mutants, a fly model of colorectal cancer, can be ameliorated by combination with ebd1[240]/Df(3L)ED9698, ebd2[136] mutant alleles.

(Tian et al., 2017)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | adult stage

(Tian et al., 2017)

increased cell number | adult stage | cell non-autonomous | somatic clone

(Tian et al., 2017)

increased cell number | adult stage | somatic clone

(Tian et al., 2017)

increased occurrence of cell division | cell non-autonomous | somatic clone

(Tian et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adult ebd1²⁴⁰ mutants show significant increase in the number of progenitor cells (intestinal stem cells and enteroblasts) in the midgut and unlike in controls frequently display chains and less frequently even clusters of progenitor cells. ebd1²⁴⁰ mutant midgut somatic clones induced during adulthood are markedly larger and contain more cells than control clones and an abnormally large number of progenitor cells (due to excessive mitotic activity of the cells) is found in the vicinity of the mutant clones.

(Tian et al., 2017)

Mutant flies have defects in the dorsal longitudinal muscles of the thorax.

(Benchabane et al., 2011)

All mutant females and a third of mutant males lack one or more dorsal longitudinal muscles in the thorax, with the remaining dorsal longitudinal muscles being increased in size.

(Benchabane et al., 2011)

ebd1²⁴⁰ mutants show a reduction in the number of a subset of dorsal longitudinal muscles, and enlargement of the remaining indirect flight muscles.

(Xin et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ebd2¹³⁶, Df(3L)ED9698, ebd1²⁴⁰ is a suppressor of increased cell number | adult stage phenotype of Apc^Q8

(Tian et al., 2017)

ebd2¹³⁶, Df(3L)ED9698, ebd1²⁴⁰ is a suppressor of abnormal cell polarity | adult stage phenotype of Apc^Q8

(Tian et al., 2017)

ebd2¹³⁶, Df(3L)ED9698, ebd1²⁴⁰ is a suppressor of abnormal cell adhesion | adult stage phenotype of Apc^Q8

(Tian et al., 2017)

ebd1²⁴⁰/ebd1²⁴⁰ is a suppressor of increased cell number | adult stage phenotype of Apc^Q8

(Tian et al., 2017)

Other

Statement

Reference

ebd1^Df5/ebd1²⁴⁰, ebd2¹³⁶/ebd2[+] has increased cell number | adult stage phenotype

(Tian et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

ebd1²⁴⁰ has indirect flight muscle cell phenotype, enhanceable by ewg[+]/ewg²

(Xin et al., 2011)

Suppressed by

Statement

Reference

Df(3L)ED9698/ebd1²⁴⁰, ebd2¹³⁶ has indirect flight muscle cell phenotype, suppressible by Scer\GAL4^ebd1.PB/Hsap\JRK^UAS.cBa

(Benchabane et al., 2011)

Suppressor of

Statement

Reference

ebd1²⁴⁰/ebd1²⁴⁰ is a suppressor of adult midgut epithelium phenotype of Apc^Q8

(Tian et al., 2017)

ebd1²⁴⁰/ebd1²⁴⁰ is a suppressor of enteroblast | increased number | adult stage phenotype of Apc^Q8

(Tian et al., 2017)

ebd2¹³⁶, Df(3L)ED9698, ebd1²⁴⁰ is a suppressor of adult midgut epithelium phenotype of Apc^Q8

(Tian et al., 2017)

ebd2¹³⁶, Df(3L)ED9698, ebd1²⁴⁰ is a suppressor of intestinal stem cell | increased number | adult stage phenotype of Apc^Q8

(Tian et al., 2017)

ebd2¹³⁶, Df(3L)ED9698, ebd1²⁴⁰ is a suppressor of enteroblast | increased number | adult stage phenotype of Apc^Q8

(Tian et al., 2017)

ebd1^Df5/ebd1²⁴⁰ is a suppressor of retina phenotype of Apc^Q8

(Benchabane et al., 2011)

ebd1²⁴⁰/Df(3L)ED4136 is a suppressor of eye photoreceptor cell phenotype of Scer\GAL4^GMR.long, arm^{S10.UAS.Tag:MYC}

(Benchabane et al., 2011)

Other

Statement

Reference

ebd1^Df5/ebd1²⁴⁰, ebd2¹³⁶/ebd2[+] has adult midgut epithelium phenotype

(Tian et al., 2017)

ebd1^Df5/ebd1²⁴⁰, ebd2¹³⁶/ebd2[+] has intestinal stem cell | increased number | adult stage phenotype

(Tian et al., 2017)

ebd1^Df5/ebd1²⁴⁰, ebd2¹³⁶/ebd2[+] has enteroblast | increased number | adult stage phenotype

(Tian et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Adult ebd1²⁴⁰/ebd1^Df5,ebd2¹³⁶ mutants show significant increase in the number of progenitor cells (intestinal stem cells and enteroblasts) in the midgut and unlike in controls frequently display chains or clusters of progenitor cells, the penetrance of this latter phenotype is increased further in ebd1^Df5,ebd2¹³⁶/ebd1²⁴⁰,ebd2¹³⁶ double mutants.

The adult midgut epithelium defects (increased number of progenitor cells, cell polarity and adhesion defects along with epithelium multi-layering) can be rescued by combination with ebd1²⁴⁰/Df(3L)ED9698,ebd2¹³⁶. The increased number of enteroblast cells in midguts of newly eclosed Apc^Q8 mutant adults is significantly suppressed by ebd1²⁴⁰ homozygosity.

(Tian et al., 2017)

ebd1²⁴⁰/ebd1^Df5 suppresses the apoptosis of photoreceptor cells which is seen in Apc^Q8 homozygotes, such that the rescued photoreceptors are wild type in length.

(Benchabane et al., 2011)

ebd1²⁴⁰/Df(3L)ED4136 suppresses the shortened photoreceptor phenotype caused by expression of arm^{S10.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.long.

(Benchabane et al., 2011)

One copy of ewg² enhances the indirect flight muscle loss seen in ebd1²⁴⁰ mutant flies.

(Xin et al., 2011)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\JRK^Scer\UAS.cBa under the control of Scer\GAL4^ebd1.PB rescues the indirect flight muscle defects seen in ebd1²⁴⁰ ebd2¹³⁶/Df(3L)ED9698 ebd2¹³⁶ mutants.

(Benchabane et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Df(3L)240

(Benchabane et al., 2011)

ebd1²⁴⁰

(Huang et al., 2021, Tian et al., 2017, Benchabane et al., 2011, Benchabane et al., 2011, Xin et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (5)

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