FlyBase Allele Report: Dmel\ebd2[136]

General Information

Symbol

Dmel\ebd2¹³⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0258668

Feature type

allele

Associated gene

Dmel\ebd2

Associated Insertion(s)

P{?EPgy2}ebd2¹³⁶

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Benchabane et al., 2011)

Progenitor genotype

P{EPgy2}ebd2^EY11662

(Benchabane et al., 2011)

Associated Insertion(s)

P{?EPgy2}ebd2¹³⁶

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 1077bp deletion from +560 to +1636 relative to the start of ebd2 translation. 477bp of the original insertion remain.

(Benchabane et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3L:21,345,118..21,346,194 [-]

Comment:

1077 bp deletion resulting from the imprecise excision of P{EPgy2}ebd2^EY11662. Extends from +560 to +1636 relative to the ebd2 start of translation. 477bp of the original insertion remains.

(Benchabane et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

The adult midgut defects (excess of mitotic cells, aberrant epithelium architecture) characteristic for Apc[Q8] mutants, a fly model of colorectal cancer, can be ameliorated by combination with ebd1[240]/Df(3L)ED9698, ebd2[136] mutant alleles.

(Tian et al., 2017)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell

(Benchabane et al., 2011, Benchabane et al., 2011)

Detailed Description

Statement

Reference

Mutant flies have defects in the dorsal longitudinal muscles of the thorax.

(Benchabane et al., 2011)

83% of mutant females show loss of dorsal longitudinal muscles of the thorax.

(Benchabane et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ebd2¹³⁶, Df(3L)ED9698, ebd1²⁴⁰ is a suppressor of increased cell number | adult stage phenotype of Apc^Q8

(Tian et al., 2017)

ebd2¹³⁶, Df(3L)ED9698, ebd1²⁴⁰ is a suppressor of abnormal cell polarity | adult stage phenotype of Apc^Q8

(Tian et al., 2017)

ebd2¹³⁶, Df(3L)ED9698, ebd1²⁴⁰ is a suppressor of abnormal cell adhesion | adult stage phenotype of Apc^Q8

(Tian et al., 2017)

Other

Statement

Reference

ebd1^Df5/ebd1²⁴⁰, ebd2¹³⁶/ebd2[+] has increased cell number | adult stage phenotype

(Tian et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Df(3L)ED9698/ebd1²⁴⁰, ebd2¹³⁶ has indirect flight muscle cell phenotype, suppressible by Scer\GAL4^ebd1.PB/Hsap\JRK^UAS.cBa

(Benchabane et al., 2011)

Suppressor of

Statement

Reference

ebd2¹³⁶, Df(3L)ED9698, ebd1²⁴⁰ is a suppressor of adult midgut epithelium phenotype of Apc^Q8

(Tian et al., 2017)

ebd2¹³⁶, Df(3L)ED9698, ebd1²⁴⁰ is a suppressor of intestinal stem cell | increased number | adult stage phenotype of Apc^Q8

(Tian et al., 2017)

ebd2¹³⁶, Df(3L)ED9698, ebd1²⁴⁰ is a suppressor of enteroblast | increased number | adult stage phenotype of Apc^Q8

(Tian et al., 2017)

Other

Statement

Reference

ebd1^Df5/ebd1²⁴⁰, ebd2¹³⁶/ebd2[+] has adult midgut epithelium phenotype

(Tian et al., 2017)

ebd1^Df5/ebd1²⁴⁰, ebd2¹³⁶/ebd2[+] has intestinal stem cell | increased number | adult stage phenotype

(Tian et al., 2017)

ebd1^Df5/ebd1²⁴⁰, ebd2¹³⁶/ebd2[+] has enteroblast | increased number | adult stage phenotype

(Tian et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Adult ebd1²⁴⁰/ebd1^Df5,ebd2¹³⁶ mutants show significant increase in the number of progenitor cells (intestinal stem cells and enteroblasts) in the midgut and unlike in controls frequently display chains or clusters of progenitor cells, the penetrance of this latter phenotype is increased further in ebd1^Df5,ebd2¹³⁶/ebd1²⁴⁰,ebd2¹³⁶ double mutants.

The adult midgut epithelium defects (increased number of progenitor cells, cell polarity and adhesion defects along with epithelium multi-layering) can be rescued by combination with ebd1²⁴⁰/Df(3L)ED9698,ebd2¹³⁶.

(Tian et al., 2017)

ebd2¹³⁶ weakly suppresses the apoptosis of photoreceptor cells which is seen in Apc^Q8 homozygotes.

(Benchabane et al., 2011)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\JRK^Scer\UAS.cBa under the control of Scer\GAL4^ebd1.PB rescues the indirect flight muscle defects seen in ebd1²⁴⁰ ebd2¹³⁶/Df(3L)ED9698 ebd2¹³⁶ mutants.

(Benchabane et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Df(3L)136

(Benchabane et al., 2011)

ebd2¹³⁶

(Tian et al., 2017, Benchabane et al., 2011, Benchabane et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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