FlyBase Allele Report: Dmel\ewg[SC3ORF.elav]

General Information

Symbol

Dmel\ewg^SC3ORF.elav

Species

D. melanogaster

Name

FlyBase ID

FBal0049487

Feature type

allele

Associated gene

Dmel\ewg

Associated Insertion(s)

Carried in Construct

P{elav-ewg.SC3ORF}

Also Known As

elav-EWG

Key Links

Transgenic product class

cDNA

(DeSimone et al., 1996)

wild_type

(DeSimone et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(DeSimone et al., 1996)

wild_type

(DeSimone et al., 1996)

Mutagen

in vitro construct

(Roy and VijayRaghavan, 1998, DeSimone et al., 1996)

Progenitor genotype

Carried in construct

P{elav-ewg.SC3ORF}

Cytology

Description

An elav promoter regulates expression of the major isoform (SC3ORF) of ewg.

(Haussmann and Soller, 2010)

Construct: ewg cDNA, SC3, with 27 nucleotides of 5' untranslated leader and 600 nucleotides of 3' trailer under the control of the elav promoter.

(DeSimone et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

elav

Encoded product / tool

ewg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with ewg²

(Xin et al., 2011)

wild-type

(DeSimone et al., 1996)

Phenotype Manifest In

indirect flight muscle cell, with ewg²

(Xin et al., 2011)

Detailed Description

Statement

Reference

Flies expressing ewg^elav.NS in a ewg² mutant background are viable but display loss of indirect flight muscles. The usual six dorsal longitudinal (DLMs) and seven dorsal ventral muscles (DVMs) on each side of the midline are all lost to be replaced by two large misshapen muscles. The photoreceptors appear normal.

(Xin et al., 2011)

Myoblasts are present, migrate normally and get distributed over the persistent thoracic larval muscles in animals expressing ewg^elav.NS in an ewg¹¹ background, but splitting of the persistent larval muscles does not occur in some genetic backgrounds. Fusion of the imaginal myoblasts with the larval muscles occurs normally, but the wild-type progressive alignment of fused myoblast nuclei into longitudinal columns does not occur in these animals.

(Roy and VijayRaghavan, 1998)

Expression can rescue embryonic lethality of ewg mutants but not the adult muscle defects.

(DeSimone et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ewg^SC3ORF.elav is a suppressor of lethal | heat sensitive phenotype of elav⁵/elav^ts1

(Haussmann et al., 2008)

ewg^SC3ORF.elav is a suppressor of abnormal neuroanatomy | heat sensitive phenotype of elav⁵/elav^ts1

(Haussmann et al., 2008)

Other

Statement

Reference

Apc^Q8, ewg^P1, ewg^SC3ORF.elav has cell lethal | adult stage phenotype

(Xin et al., 2011)

elav⁵/elav^ts1, ewg^SC3ORF.elav has flightless phenotype

(Haussmann et al., 2008)

Phenotype Manifest In

Suppressor of

Statement

Reference

ewg^SC3ORF.elav is a suppressor of NMJ bouton | heat sensitive phenotype of elav⁵/elav^ts1

(Haussmann et al., 2008)

ewg^SC3ORF.elav is a suppressor of neuromuscular junction | heat sensitive phenotype of elav⁵/elav^ts1

(Haussmann et al., 2008)

Other

Statement

Reference

Apc^Q8, ewg^P1, ewg^SC3ORF.elav has photoreceptor | adult stage phenotype

(Xin et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Hemizygous ewg^P1 suppresses the apoptosis seen in Apc^Q8 mutant photoreceptors. This apoptosis can be restored by expressing ewg^elav.NS.

(Xin et al., 2011)

elav^ts1/elav⁵ animals eclose as adults when they are rescued by ewg^elav.NS (providing that embryogenesis occurs at the permissive temperature for the elav^ts1 allele). However, the rescued adults show motor defects and are flightless.

The reduction in bouton number at the neuromuscular junction which is seen in elav^ts1/elav⁵ animals reared at the restrictive temperature during larval life is rescued by ewg^elav.NS.

(Haussmann et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

ewg^SC3ORF.elav rescues ewg¹⁰

(DeSimone et al., 1996)

ewg^SC3ORF.elav rescues ewg¹¹

(DeSimone et al., 1996)

ewg^SC3ORF.elav rescues ewg⁵

(DeSimone et al., 1996)

Partially rescues

ewg^SC3ORF.elav partially rescues ewg¹/ewg²

(DeSimone et al., 1996)

Comments

Expression of ewg^elav.NS rescues the embryonic lethality seen in ewg² mutants.

(Xin et al., 2011)

Expression of ewg^SC3ORF.elav rescues the viability of ewg² mutants.

(Haussmann and Soller, 2010)

The increase in bouton number seen at the larval neuromuscular junctions of ewg² motorneurons is partially rescued by ewg^elav.NS.

(Haussmann et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

NS⁴

(Roy and VijayRaghavan, 1998)

ewg^SC3ORF.elav

ewg^elav.NS

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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