Isolation and characterization of Df(3R)BSC846
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC846 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG14509f04904 and P{XP}d00146. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG14509f04904/P{XP}d00146 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC846 from the segment of PBac{WH}CG14509f04904 to the left of its FRT site and the segment of P{XP}d00146 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3R)BSC846 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3R:25017393 ;25589319 and the cytological breakpoints predicted from these coordinates are 99A1;99B10. Df(3R)BSC846 failed to complement stg4.