Isolation and characterization of Df(2L)BSC855
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC855 was isolated as a FLP recombinase-induced recombination event involving P{XP}d05987 and PBac{WH}CG9426f05662. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d05987/PBac{WH}CG9426f05662 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC855 from the segment of P{XP}d05987 to the left of its FRT site and the segment of PBac{WH}CG9426f05662 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(2L)BSC855 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2L:12832803 ;13219548 and the cytological breakpoints predicted from these coordinates are 34A1;34A10. Df(2L)BSC855 failed to complement Vha68-2s4214.