We used information provided by Carlos Flores & Bill Engels, University of Wisconsin, Madison, with stocks donated to the BDSC to estimate release 5 sequence coordinates for deficiencies carried in those stocks. These are their statements (from March 2004) followed by our estimates for each deficiency. FBab0026816 Df(2R)50C-102 "Deletes from the middle of Cp1 proximally 10.2 kb into the longer transcripts of AGO1." Estimated breakpoints in R5 sequence coordinates: 2R:9841630 ;9851830 R5 cytology corresponding to estimated sequence coordinates: 50C16;50C19 FBab0026821 Df(2R)50C-129 "Deletes from the middle of Cp1 distally 27 kb to near the start of longer mam transcripts." Estimated breakpoints in R5 sequence coordinates: 2R:9851830 ;9878830 R5 cytology corresponding to estimated sequence coordinates: 50C19;50C23 FBab0026827 Df(2R)50C-36 "Deletes from the middle of Cp1 distally 43 kb into mam." Estimated breakpoints in R5 sequence coordinates: 2R:9851830 ;9894830 R5 cytology corresponding to estimated sequence coordinates: 50C19;50C23 FBab0026828 Df(2R)50C-38 "Deletes from the middle of Cp1 distally 211 kb into cg." Estimated breakpoints in R5 sequence coordinates: 2R:9851830 ;10062830 R5 cytology corresponding to estimated sequence coordinates: 50C19;50E1