Isolation and characterization of Df(1)BSC867
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC867 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}ogref07788 and P{XP}CG14427d06860. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118 PBac{WH}ogref07788/w1118 P{XP}CG14427d06860; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC867 from the segment of PBac{WH}ogref07788 to the left of its FRT site and the segment of P{XP}CG14427d06860 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(1)BSC867 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  X:6875892 ;6935548 and the cytological breakpoints predicted from these coordinates are 6E4;6F1. To confirm the presence of the deficiency, we showed that a 883 base pair fragment spanning the PBac{WH}cmf05774 insertion site could not be amplified from Df(1)BSC867/PBac{WH}cmf05774 flies using primers 5'-CCACATAGTTGTCCTTGATCACGG-3' and 5'-CCGTGTTTGTGTATTGCCTCCGTCAC-3'.