Isolation and characterization of Df(1)BSC870
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC870 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f02395 and P{XP}jogd06025. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118 PBac{WH}f02395/w1118 P{XP}jogd06025; MKRS,P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC870 from the segment of PBac{WH}f02395 to the left of its FRT site and the segment of P{XP}jogd06025 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(1)BSC870 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  X:19584121 ;19742393 and the cytological breakpoints predicted from these coordinates are 18E1;18F3. To confirm the presence of the deficiency, we showed that a 367 base pair fragment spanning the P{SUPor-P}CG12703KG08105 insertion site could not be amplified from Df(1)BSC870/P{SUPor-P}CG12703KG08105 flies using primers 5'-GTGATAACTTGTTCGATAGCTCTTCG-3' and 5'-CTTCCTGTACTACAACTGGTGGTGG-3'.