Isolation and characterization of Df(1)BSC872
Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC872 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG14235f04041 and P{XP}d06197. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w1118 PBac{WH}CG14235f04041/w1118 P{XP}d06197; MKRS, P{hsFLP}86E/+ females crossed to FM7h/Dp(2;Y)G, P{hs-hid}Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC872 from the segment of PBac{WH}CG14235f04041 to the left of its FRT site and the segment of P{XP}d06197 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d06197 to be Release 3 genomic coordinate 20003203 on the X chromosome, which corresponds to  X:20201432  on the Release 5 map. The predicted cytology of both coordinates is 19C3. PBac{WH}CG14235f04041 maps to Release 5 coordinate  X:19638713  with predicted cytology 18E5. Consequently, the breakpoints of Df(1)BSC872 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  X:19638713 ;20201432 and the cytological breakpoints predicted from these coordinates are 18E5;19C3. It failed to complement sw1.