Isolation and characterization of Df(3R)BSC873
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC873 was isolated as a FLP recombinase-induced recombination event involving P{XP}d04850 and PBac{RB}CG18749e03518. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d04850/PBac{RB}CG18749e03518 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). The recombination event generated the genetic element P+PBac{XP3.RB5}BSC873 from the segment of P{XP}d04850 to the left of its FRT site and the segment of PBac{RB}CG18749e03518 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d04850 to be Release 3 genomic coordinate 3591234 on chromosome arm 3R, which corresponds to Release 5 coordinate  3R:3591219 . The predicted cytology of both coordinates is 84E1. PBac{RB}CG18749e03518 maps to Release 5 coordinate  3R:4069851  with predicted cytology 84F5. Consequently, the breakpoints of Df(3R)BSC873 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3R:3591219 ;4069851 and the cytological breakpoints predicted from these coordinates are 84E1;84F5. Df(3R)BSC873 failed to complement grnH10.