We are providing prepublication data from our lab that we would be happy to see presented to the community by FlyBase. These data may be augmented by further data prior to publication and by reanalysis that could include base calling, alignment, and other changes. We sequenced poly A+ mRNA from heads of Drosophila pseudoobscura females and males. The dataset consists of reads that pass default parameters of the Illumnia pipeline: 63 million reads for female and 71 million reads for males. Reads were mapped with Bowtie 0.11.3 against the portion of Flybase r2.6 that contained sequences aligned to chromosomes for D. pseudoobscura using -v 2 -m 1. Additional details regarding experimental procedures can be found at Gene Expression Omnibus Series Number GSE19989. These data are not yet fully integrated with other FlyBase data. Datasets (labels are interim): Male_Heads - 71 million reads Female_Heads - 63 million reads