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Daines, B., Emmert, D., Gelbart, W., Chen, R. (2011.3.30). Addendum concerning analysis of RNA-Seq junctions 
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FBrf0213348
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A small number of junctions predicting very small and very long introns may be mispredictions and should be interpreted with care; these are currently being re-examined.  In addition, for ~1900 predicted junctions, the supporting evidence came from a methodology (Wang L, Xi Y, Yu J, Dong L, Yen L, et al. 2010 A Statistical Method for  the Detection of Alternative Splicing Using RNA-Seq. PLoS ONE 5(1):  e8529. doi:10.1371/journal.pone.0008529) that did not report number of supporting junction-spanning reads.  In reality, each of these ~1900 predicted junctions was supported by at least two reads, and so we have noted these by having a total junction-spanning read count of ">1".  However, we do not know which developmental stages these supporting reads were in, and so all developmental stages are reported as "0".
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    English
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