Cell lines donated by Amanda Simcox. 1) Rumi 4 (rumi26, UAS-RasV12/ rumi26, Act5C-Gal4). The generation of the lines is described in Simcox et al 2008 (http://www.landesbioscience.com/journals/fly/article/7427/). 2-3) Ras H3 and Ras H7 (UAS-GFP; UAS-RasV12/ Act5C-Gal4). These are unpublished lines but were generated by the method in Simcox et al 2008 (http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1000142). 4) wts Ras (UAS-GFP, UASwtsRNAi; UAS-RasV12/ Act5C-Gal4). This line is epithelial.