Kirino, Y., Vourekas, A., Khandros, E., Mourelatos, Z. (2011). Immunoprecipitation of piRNPs and directional, next generation sequencing of piRNAs. Methods Mol. Biol. (N.Y.) 725(): 281--293.
FlyBase ID
FBrf0214652
Publication Type
Research paper
Abstract
Piwi interacting RNAs (piRNAs) are small (∼25 to ∼30 nucleotide) and are expressed in the germline. piRNAs bind to the Piwi subclade of Argonaute proteins and form the core ribonucleoproteins (RNPs) of piRNPs. We describe a method for the massive identification of piRNAs from immunopurified piRNPs. This strategy may also be used for immunopurification and directional sequencing of RNAs from other RNPs that contain small RNAs.
