We sequenced cDNAs derived from poly A+ mRNA of male and female adults (aged 5-7 days post-eclosion) of D. melanogaster, D. simulans, D. yakuba, D. ananassae, D. pseudobscura, D. mojavensis, and D. virilis. Details regarding experimental procedures can be found at Gene Expression Omnibus Series Number GSE28078 and Zhang et al. 2007 (Nature  450:233-237 ). For D. melanogaster, D. mojavensis, D. pseudoobscura, and D. simulans, both sexes are represented. The DSSC stock numbers for strains used are listed below (except for D. melanogaster, for which the genotype is listed):
D. ananassae  14024-0371.13
D. melanogaster  y1 w67c
D. mojavensis  15081-1352.22
D. pseudoobscura  14011-0121.94
D. simulans  14021-0251.011 and 14021-0251.198
D. virilis  15010-1051.87
D. yakuba  14021-0261.01
Data set | number of junctions (>3 offset reads) | number of uniquely aligned reads:
Dana_371.13_F	|	44,958	|	80,795,399
Dmel_y1w67c_F	|	42,703	|	74,088,186
Dmel_y1w67c_M	|	51,030	|	73,035,115
Dmoj_1352.22_F	|	43,377	|	77,551,101
Dmoj_1352.22_M	|	50,614	|	82,643,665
Dpse_111.94_F	|	38,897	|	42,364,942
Dpse_111.94_M	|	47,517	|	65,254,228
Dsim_251.011_F	|	39,039	|	50,066,324
Dsim_251.011_M	|	45,733	|	63,900,328
Dsim_251.198_F	|	39,731	|	60,952,087
Dsim_251.198_M	|	40,527	|	38,628,414
Dvir_1051.87_F	|	51,858	|	118,838,220
Dyak_261.01_F	|	42,605	|	75,484,498