FlyBase Reference Report: Shah and Carbonetto, 2012.5.22, hDAG1 construct and insert from Salvatore Carbonetto's lab.

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Citation

Shah, W.A., Carbonetto, S. (2012.5.22). hDAG1 construct and insert from Salvatore Carbonetto's lab.

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FBrf0218454

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Personal communication to FlyBase

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Text of Personal Communication

The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Waris Ali Shah and Salvatore Carbonetto, McGill University.
P{UAS-hDAG1.C} consists of human DAG1 (dystroglycan 1/dystrophin-associated glycoprotein 1) sequences under the control of UAS.Human dystroglycan was amplified from OpenBiosystem clone#MHS1011-75229 using the primers below and the PCR product was cloned into pUAST as a BglII-NotI fragment.
Sense primer: 5prime-GCGCTCGAGATCTATGAGGATGTCTGTGGGCC-3prime
Antisense primer: 5prime-ATTGCGGCCGCTTAAGGTGGGACATAGGGAGG-3prime
P{UAS-hDAG1.C}2 is a viable and fertile insertion on the second chromosome.

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English

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