FB2026_02 , released June 18, 2026
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Citation
Hou, X., Pedi, L., Diver, M.M., Long, S.B. (2012). Crystal structure of the calcium release-activated calcium channel Orai.  Science 338(6112): 1308--1313.
FlyBase ID
FBrf0220161
Publication Type
Research paper
Abstract
The plasma membrane protein Orai forms the pore of the calcium release-activated calcium (CRAC) channel and generates sustained cytosolic calcium signals when triggered by depletion of calcium from the endoplasmic reticulum. The crystal structure of Orai from Drosophila melanogaster, determined at 3.35 angstrom resolution, reveals that the calcium channel is composed of a hexameric assembly of Orai subunits arranged around a central ion pore. The pore traverses the membrane and extends into the cytosol. A ring of glutamate residues on its extracellular side forms the selectivity filter. A basic region near the intracellular side can bind anions that may stabilize the closed state. The architecture of the channel differs markedly from other ion channels and gives insight into the principles of selective calcium permeation and gating.
PubMed ID
23180775
PubMed Central ID
PMC3695727 (PMC) (EuropePMC)
DOI
10.1126/science.1228757
Related Publication(s)
Note

Orai channel pore properties and gating by STIM: implications from the Orai crystal structure.
Rothberg et al., 2013, Sci. Signal. 6(267): pe9 [FBrf0222668]

CRACking the structure of Orai: new work reveals the intramolecular properties of a store-operated, calcium influx channel.
Braun, 2013, Channels 7(2): 71--73 [FBrf0223569]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Science
    Title
    Science
    Publication Year
    1895-
    ISBN/ISSN
    0036-8075 1095-9203
    Data From Reference
    Genes (1)
    Physical Interactions (1)