The following information accompanied stocks donated to the Bloomington
Drosophila Stock Center by Douglas Kim, Howard Hughes Medical Institute,
Janelia Farm Research Campus.
P{13XLexAop2-IVS-GCaMP6s-SV40},
P{13XLexAop2-IVS-GCaMP6s-p10},
P{13XLexAop2-IVS-GCaMP6m-p10},
P{13XLexAop2-IVS-GCaMP6m-SV40},
and P{13XLexAop2-IVS-GCaMP6f-p10}
encode high sensitivity green fluorescent calcium sensor protein variants under
the control of 13 copies of the LexA operator (see Pfeiffer et al.,
2010, FBrf0212052 for information on similar vectors).
Variant names are based on decay kinetics -- GCaMP6s (slow), GCaMP6m
(medium), and GCaMP6f (fast). In a Drosophila larval neuromuscular
junction stimulation and imaging assay, they perform as follows relative
to GCaMP5G (for GCaMP5G kinetics see Akerboom et al., 2012, FBrf0219564):
construct | 1 Hz deltaF/F0 | 5 Hz deltaF/F0 | 20 Hz deltaF/F0 | 80 Hz deltaF/F0 | 160 Hz deltaF/F0 | Decay time 20 Hz
GCaMP5G | 100% | 100% | 100% | 100% | 100% | 100%
GCaMP6s | 216% | 585% | 370% | 134% | 121% | 188%
GCaMP6m | 358% | 1079% | 419% | 112% | 104% | 144%
GCaMP6f | 160% | 213% | 175% | 111% | 113% | 62%
P{13XLexAop2-IVS-GCaMP6s-SV40}su(Hw)attP1 is a homozygous viable and
fertile insertion on the third chromosome in an attP site at 87B13,
3R:8299797..8303038 .
P{13XLexAop2-IVS-GCaMP6s-SV40}su(Hw)attP5 is a homozygous viable and
fertile insertion on the second chromosome in an attP site at 50F1,
2R:10194873..10195195 .
P{13XLexAop2-IVS-GCaMP6s-p10}su(Hw)attP1 is a homozygous viable and
fertile insertion on the third chromosome in an attP site at 87B13,
3R:8299797..8303038 .
P{13XLexAop2-IVS-GCaMP6s-p10}su(Hw)attP5 is a homozygous viable and
fertile insertion on the second chromosome in an attP site at 50F1,
2R:10194873..10195195 .
P{13XLexAop2-IVS-GCaMP6m-p10}su(Hw)attP1 is a homozygous viable and
fertile insertion on the third chromosome in an attP site at 87B13,
3R:8299797..8303038 .
P{13XLexAop2-IVS-GCaMP6m-SV40}su(Hw)attP1 is a homozygous viable and
fertile insertion on the third chromosome in an attP site at 87B13,
3R:8299797..8303038 .
PBac{13XLexAop2-IVS-GCaMP6m-p10}VK00005 is a homozygous viable and fertile
insertion on the third chromosome in an attP site at 75A10, 3L:17945208 .
P{13XLexAop2-IVS-GCaMP6f-p10}su(Hw)attP5 is a homozygous viable and
fertile insertion on the second chromosome in an attP site at 50F1,
2R:10194873..10195195 .
--
Annette Parks, Ph.D.
Bloomington Drosophila Stock Center
http://flystocks.bio.indiana.edu
