Piñeyro, D., Blanch, M., Badal, M., Kosoy, A., Bernués, J. (2013). GAGA factor repression of transcription is a rare event but the negative regulation of Trl is conserved in Drosophila species. Biochim. Biophys. Acta 1829(10): 1056--1065.
FlyBase ID
FBrf0222669
Publication Type
Research paper
Abstract
GAGA is a highly conserved Drosophila transcription factor encoded by the Trithorax-like (Trl) gene. While GAGA usually activates transcription, it represses its own promoter. Here we show that GAGA-mediated repression of Trl is conserved between two distant Drosophila species. A detailed promoter study showed that GAGA repressive activity can't be attributed to any discrete element in the Trl promoter. Genome-wide analysis of the transcriptome in S2 cells indicated that repression of Trl is very likely unique, being GAGA factor a transactivator for all the other promoters. Taken together, our results suggest a new mechanism to explain GAGA-mediated repression that involves a dose-dependent change in the architecture of the Trl promoter.
