The following information accompanied stocks donated to the Bloomington Stock Center by Kent Nybakken, Boston Biomedical Research Institute.
To produce P{UAS-betaCOP.HA}, the full length coding sequence from betaCOP (FBgn0008635), lacking only the C-terminal stop codon, was PCR'ed using primers engineered to produce a 5' in-frame Bgl II site and an in-frame 3' Not I site. This fragment was digested with Bgl II and Not I and ligated into the Bgl II and Not I sites of a vector made by Kent Nybakken containing an in-frame, C-terminal, triple hemagglutinin (HA) tag followed by a stop codon. The C-terminal triple HA-tagged form of betaCOP was excised by cleavage with Bgl II and Xba I and ligated into a similarly cut pP{UAST} vector.
P{UAS-betaCOP.HA}10M is a homozygous and hemizygous viable and fertile, X chromosome insertion.
P{UAS-betaCOP.HA}6M is a homozygous viable and fertile, second chromosome insertion.
P{UAS-betaCOP.HA}1M is a third chromosome insertion.