The following information accompanied stocks donated to the Bloomington Stock Center by Balaji Iyengar, Hamilton Health Sciences.
P{UAS-DD.mCherry.I} expresses mCherry tagged at the N-terminus with the destabilization domain of human FKBP12 described in Banaszynski et al. (2006) "A Rapid, Reversible, and Tunable Method to Regulate Protein Function in Living Cells Using Synthetic Small Molecules", Cell 126: 995–1004. Degradation of the mCherry fusion protein was inhibited in vivo by feeding larvae the small molecule inhibitor Shield-1. The construct was based on pP{UAST}.
P{UAS-DD.mCherry.I}2 is a homozygous viable and fertile, second chromosome insertion.