The following information accompanied stocks donated to the Bloomington Stock Center by Kent Nybakken, Boston Biomedical Research Institute.
P{UAS-Pp2A-29B.HA} was generated by PCR amplifying the coding region of Pp2A-29B (FBgn0260439) from the LD10247 cDNA using oligonucleotide primers with in-frame 5' Bam HI and 3' Not I sites. These sites were cut and Pp2A-29B (without the stop codon) was subcloned into the Bam HI and Not I sites of a vector made by Kent Nybakken containing an in-frame, C-terminal, triple hemagglutinin (HA) tag followed by a stop codon. The triple-HA tagged Pp2A-29B construct was then excised with Bgl II and Xba I and directionally cloned into the Bgl II and Xba I sites of pP{UAST}. The final arrangement of selected features is Bgl II--Eco RV--Pp2A-29B full-length codons--Not I--triple HA--stop codon--Sma I--Xba I.
P{UAS-Pp2A-29B.HA}4M is a homozygous and hemizygous viable and fertile, X chromosome insertion.
P{UAS-Pp2A-29B.HA}3M is a second chromosome insertion.
P{UAS-Pp2A-29B.HA}6F is a third chromosome insertion.