Here is the spreadsheet of RNA-Seq datasets with the requested metadata. Notes: 1. The 6 of the 30 BAM files listed in the file are for 8 new species, while 2 BAM files for Dana, and 4 BAM files for Dmel are missing. I replaced the 6 rows for 8 new species with 6 for Dana and Dmel. 2. For heads data, there are GAI and GAII samples in GEO. We only use GAII sample to make BAM file. I have deleted GAI sample accessions (GSM). 3. Raw reads were trimmed to 75 nt before mapping. 36 nt reads in Dpse heads samples were removed. Therefore, all the reads for mapping are 75 nt. 4. We used TopHat (v2.0.8b) to do the mapping. For most samples, we used parameters: -g 1 -r 150 --solexa1.3-quals; For head data, we used: -g 1 -r 150 Best, Zhenxia
