The following information accompanied stocks donated to the Bloomington Stock Center by Sharyn Endow, Duke University Medical Center.
P{ncd-Eb1.GFP} was described in Liang et al. (FBrf0206773). It consists of the ncd promoter fused to Eb1 cDNA followed by sequences encoding the three amino acid linker GLK and the coding region for the S65T variant of GFP in a miniwhite-marked vector.
The Eb1 sequence preceding GLK is
MAVNVYSTNVTSENLSRHDMLAWVNDCLQSQFSKIEELCTGAAYCQFMDMLFPNSVPVKRVKFRTNLEHEYIQNFKILQAGFKKMSVDKIIPIDKLVKGRFQDNFEFLQWFKKFFDANYDGRDYDASAVREGAPMGFGSGAVKSLPGTAASGVSSSYRRGPSATTRPAMTSAVKPTVSKVLPRTNNAAPASRINACANSTGTVKKNDVSNSVNNQQIEEMSNQVMDMRINLEGLEKERDFYFSKLRDIEILCQEADDAEAHPIIQKILDILYATEDGFAPPDDAPPEDEEY
P{ncd-Eb1.GFP}M6F1 is a homozygous and hemizygous viable and fertile, X chromosome insertion.
P{ncd-Eb1.GFP}M1F3 is a third chromosome insertion.