Sarou-Kanian, V., Joudiou, N., Louat, F., Yon, M., Szeremeta, F., Même, S., Massiot, D., Decoville, M., Fayon, F., Beloeil, J.C. (2015). Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR.  Sci. Rep. 5(): 9872.

FBrf0228222

Research paper

We have developed new methods enabling in vivo localization and identification of metabolites through their (1)H NMR signatures, in a drosophila. Metabolic profiles in localized regions were obtained using HR-MAS Slice Localized Spectroscopy and Chemical Shift Imaging at high magnetic fields. These methods enabled measurement of metabolite contents in anatomic regions of the fly, demonstrated by a decrease in β-alanine signals in the thorax of flies showing muscle degeneration.

PMC4402646 (PMC) (EuropePMC)