Here you have the primer sequences for the generation of the knock-out (KO) allele described in
Merino et al., 2015, Cell 160(3): 461--476. (FBrf0227415)
There are two sequences 5’ Arm and 3’ Arm. Between these two homologous arms genomic regions are deleted for the generation of azotKO. Note that the sequences of the arms are present in the KO.
Azot Knock-Out.
5’ Arm:
Fwrd cg11165 5’arm-SacII
5’ AACCGCGGCAACGCAATACAAAATACCCCGC 3’
Rev cg11165 5’arm-KpnI
5’ AAGGTACCGGACGGACAGGCAGACGGAA 3’
3’ arm:
Fwrd cg11165 3’arm-AscI
5’ AAGGCGCGCCGAGCGGCAGTTTGACCGAGG 3’
Revcg11165 3’arm-PstI
5’ AACTGCAGTTTGGGGGTCTTGAGTGCCG 3’