Schlötterer, C., Kofler, R., Versace, E., Tobler, R., Franssen, S.U. (2015). Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing genetic variation.  Heredity 114(5): 431--440.

FBrf0228791

Review

Evolve and resequence (E&R) is a new approach to investigate the genomic responses to selection during experimental evolution. By using whole genome sequencing of pools of individuals (Pool-Seq), this method can identify selected variants in controlled and replicable experimental settings. Reviewing the current state of the field, we show that E&R can be powerful enough to identify causative genes and possibly even single-nucleotide polymorphisms. We also discuss how the experimental design and the complexity of the trait could result in a large number of false positive candidates. We suggest experimental and analytical strategies to maximize the power of E&R to uncover the genotype-phenotype link and serve as an important research tool for a broad range of evolutionary questions.

PMC4815507 (PMC) (EuropePMC)