FB2026_03 , released September 17, 2026
Reference Report
Open Close
Reference
Citation
Levis, R. (2015.10.28). l(2)10408 insertion site sequence location. 
FlyBase ID
FBrf0230293
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication
In FlyBase release FB2015_05, the Gbrowse triangle for P{PZ}l(2)35Bd10408 (FBti0004833) is shown within the l(2)35Bc gene, while l(2)10408 is annotated as an allele of the neighboring l(2)35Bd gene.  The insertion site release 6 genome sequence location in FlyBase is recorded as  2L:15009505..15010016 .  This range of values is based on the alignment of the flanking sequence for l(2)10408 (GBacc AQ073297).  The comment in the GenBank record is that this sequence is recovered from the 5’ flank of the P element by plasmid rescue and that the insertion site is unknown within the 534 bp flank.  However, when I looked at this strain in the LBNL GDP database, I saw that the insertion site is recorded as being at position 24 within the 534 bp.  Bases 1-16 align with the canonical sequence at the 5’ end of the P element and bases 17-530 align with the release 6 genome sequence segment  2L:15010016..15009504 .  The insertion site is 24 rather than 17 because of the convention we use to adjust the 5’ flank coordinate for the 8 bp target-site duplication.  This puts the insertion site sequence location at  2L:15010009..15010009 .  This site is at +70 bp within the l(2)35Bd gene.
DOI
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference
    Insertions (1)