The sequences surrounding the Rab3-GEFSC225 excision mutant are as follows: ccaaaataaaggaccttcattaaaagaaagaggttca – Excision – ccttgaagccgaagaatgaatgattgttgtctggcatt I can confirm that the 2115 aa Rab3-GEF isoform that we reported matches Rab3-GEF-PJ, newly annotated in FlyBase based on the information in the paper.