FB2026_03 , released September 17, 2026
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Pindyurin, A., van Steensel, B. (2016.7.25). HP1 inducible DamID constructs and insertions. 
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FBrf0233308
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Personal communication to FlyBase
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The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Alexey Pindyurin and Bas van Steensel.
M{hs-dam.4-HT-intein-L127C-HP1} expresses a Dam-Myc-HP1 fusion protein under the control of the full-length hsp70 promoter.  The Dam ORF contains the 4-hydroxytamoxifen-inducible intein (Buskirk et al, 2004, PNAS,  101:10505--10510 ) inserted immediately prior to (mutated) position L127C. Insertion of the intein results in reduced activity of the Dam protein.
M{hs.min(FRT.STOP1)dam-HP1} can express a Dam-Myc-HP1 fusion protein (under the control of the minimal hsp70 promoter) after FLP-mediated excision of the FRT-STOP#1-FRT cassette.  The STOP#1 transcription termination sequence consists of the C-terminal sequence of the yeast HIS3 gene, the SV40 polyadenylation signal, a false translation initiation signal and a 5' splice donor site.
M{hs-dam.4-HT-intein-L127C-HP1}ZH-51C is an insertion into M{3xP3-RFP.attP'}ZH-51C.
M{hs.min(FRT.STOP1)dam-HP1}ZH-51C is an insertion into M{3xP3-RFP.attP'}ZH-51C.
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    English
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    Alleles (2)
    Genes (2)
    Natural transposons (1)
    Insertions (3)
    Experimental Tools (4)
    Transgenic Constructs (2)