FlyBase and the BDSC both currently have the sequence location of P{lacW}l(1)G0272G0272 (FBti0013149) as X:16 ,447,340..16,447,611 +. Ulrich Schaefer submitted a 400 bp flanking sequence that was determined using 3' inverse PCR. The first ~150 bp of this sequence have N's scattered through it. When I align the entire 400 bp to the release 6 reference sequence, I get a credible alignment of the segment from 20-400 to X:16447236-16447611 (+) (see below). Because this is a 3' flank, that would put the insertion site at X:16447216..16447236 , which is ~100 bp to the left of the range currently given by FlyBase and the BDSC.
This places the insertion site ~ 223-243 bp upstream of the 5' end of the eIF2alpha gene.
Plus Strand HSP:
Score = 1345 (374 bits), P = 0
Identities = 340/381 (89.2%), Positives = 340/381 (89.2%), Strand = Plus / Plus
Query: 20 ATGTTNATGTTTGTTAAANTTTATTGCGGTNAAANAAAANTTGCGATAATANCCCNATGA 79
||||| || ||||||||| |||||||| | | |||| |||||| ||| || | |
Sbjct: 16447236 ATGTTAATTTTTGTTAAA-TTTATTGCAATATTACAAAAATTGCGAAAATTGACCCAAAA 16447294
Query: 80 GTTNAATTCTCGAAAAGTTATATAGATTGCTAATACTGNATATATNAGGAATAAAAAGCA 139
||| ||||||| |||||||||||||||||||||||||| ||| || ||||||||||||||
Sbjct: 16447295 GTTAAATTCTCAAAAAGTTATATAGATTGCTAATACTG-ATAAAT-AGGAATAAAAAGCA 16447352
Query: 140 GTTGTTAAGTTATCAATATGTCACTTTTGAGCCAAGTTATGATGATAGTTCCCATAAGTC 199
|||||| ||||| ||||||||||||| | ||| |||||||||||| |||||||| |||
Sbjct: 16447353 GTTGTTCTTTTATCTATATGTCACTTTTTATCCATGTTATGATGATAATTCCCATATGTC 16447412
Query: 200 AAATGTTCATTTTTCATATTTATCTGTTTAAATTTGCAGTAACATTTTAAAAGGTTGCAT 259
|||| |||||||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct: 16447413 AAATTTTCATTTTTCATATTTATCTGTTTAAATTTGCAG-AACATTTTAAAAGGTTGCAT 16447471
Query: 260 ATAATTCGCAAACAAAAAGGCCATTAAATATGTAATGACATGTAGCTTAAATCGGTTTTT 319
|| | ||||| ||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct: 16447472 ATTA-TCGCATACAAAAAGGCCATTAAATATGTAATGACATGTAGCTTGAATCGGTTTTT 16447530
Query: 320 TTAGAAGCTTCTCTTTTAATTTAAAGAACTTTAGAAAATTTGTATAGGTTGGCTCCGTTT 379
||||||||| | ||||||||||||||||| |||||| |||||||||||||||||||||||
Sbjct: 16447531 TTAGAAGCTACACTTTTAATTTAAAGAACATTAGAATATTTGTATAGGTTGGCTCCGTTT 16447590
Query: 380 ATTAGTTAATTATGACAGCGC 400
|||||||||||||||||||||
Sbjct: 16447591 ATTAGTTAATTATGACAGCGC 16447611