The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Tamas Lukacsovich and Larry Marsh, University of California, Irvine.
M{UAS-HTT.RT1.Q25} is composed of the first exon of Hsap\HTT with 25 CAG repeats plus ~140 bp of the first intron under the control of UAS. The intron includes both the stop codon at the exon/intron boundary and the next in-frame stop codon.
M{UAS-HTT.RT1.Q25}ZH-51D is a homozygous viable and fertile insertion on the second chromosome in the ZH-51D attP site at 51D9,  2R:15054298 .
M{UAS-HTT.RT2.Q25} is composed of the first exon of Hsap\HTT with 25 CAG repeats plus ~140 bp of the first intron under the control of UAS. The first stop codon in the intron (at the exon/intron boundary) has been mutated to R allowing readthrough to the second in-frame stop codon. Readthrough adds an additional 39 amino acids and results in the following protein: MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPRVWARCSSLSRRVPGYGGDGGNPAACGPATRTPGPAETE*.
M{UAS-HTT.RT2.Q25}ZH-51D is a homozygous viable insertion on the second chromosome in the ZH-51D attP site at 51D9,  2R:15054298 .
M{UAS-HTT.RT3.Q120} is composed of the first exon of Hsap\HTT with 120 CAG repeats plus ~140 bp of the first intron under the control of UAS. The intron includes both the stop codon at the exon/intron boundary and the next in-frame stop codon.
M{UAS-HTT.RT3.Q120}ZH-51D is a homozygous viable and fertile insertion on the second chromosome in the ZH-51D attP site at 51D9,  2R:15054298 .
M{UAS-HTT.RT4.Q120} is composed of the first exon of Hsap\HTT with 120 CAG repeats plus ~140 bp of the first intron under the control of UAS. The first stop codon in the intron (at the exon/intron boundary) has been mutated to R allowing readthrough to the second in-frame stop codon. Readthrough adds an additional 39 amino acids.
M{UAS-HTT.RT4.Q120}ZH-51D is a homozygous viable and fertile insertion on the second chromosome in the ZH-51D attP site at 51D9,  2R:15054298 .