FB2026_03 , released September 17, 2026
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Brown, B., Ryoo, H.D. (2018.6.27). Location data for the hiro[F1] deletion. 
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FBrf0239281
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Personal communication to FlyBase
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Text of Personal Communication 
The hiroF1 allele was created by targeted homologous recombination. The gRNAs/HDR template were designed to cut from position  3R:686 ,041 through  3R:687 ,368 and the sequence was replaced with the 3xP3>dsRed-attP cassette. Below is the sequence of the deleted region:
> 3L:686041..687368 
tatcggcaggaaccatgtgaccagcccggttgacccaaaacatgctgaagttgccatagg
tcttctcgtaaccctcgagaactcgatctacggtgattcccacgcgcggagagacctggt
aagatggcttggcggaccattccattgcttcgatccagttgacggctcccggagtggcgc
aaatcaggtccagtcctccggagaacacgcccacttttacggtggtgttgttcagcagtt
cacccactaaaagtgtcataaggctataatttgaggtagcacccagacggttgaaacata
ccaacatccacagctggcttcatgaaatcgcccatcagcttggtgaaggtggtgccgctc
tgggcgccccatttcactccggtgttgattccgagggcctcggtcacgggtccaagcatt
agatcctccagcaatttgtcgcggtcctcatccacatcgaatttcacgagggtgcgatac
atgacctctggataagtggttgatgagaatcagctcggtaagttacgcaacggatcttac
cctccggagtcatggcagctctggacatcaacctgtactgatcacccaatgttggggtct
ccacgttatagaagtccactcccttggactcgcgcagcaccactgactgggtggagctcc
actgcaaagtggcctgtgtccacttctcgcggtcaacatagtccttagtcttcagggccg
aagcttcaatcttgtcatggccatcctgatccacgattcccaactgcagcaggaagggag
cccacgagagcacagagtctatgggtgaggtccagggatctcccaaggccaccgaaacaa
agttgctctcgatctcaccgcgcttgatggcgtaatccaactccaaagcgaactccgggg
ccatctttccgccataactctcgcagaagatgtgcagcggcacagtcttgaattccgggt
ggtttgtgtagaagcccttcatcagctcaacgagatccagggcgatctgcttgttgttgg
tcgtgtagtaagaagatccgtccacgtagctgaaaccactgcccacgggattgtcgatga
acatcacgttcatgtccttcacccaagtccagtcgcggtagctgccgtcgagcttcaggg
gacccaactcctcaaagtttccgtatcccgttgaggaggcacctggacctccttggagcc
aaatggccagcggacgctccgtgtaactggagacattggcggtggtgtagtacagccagt
agaacatgtgggcgccagtcctgacatccacaaatccccaatcctgatcaccgggtccat
agcccttg
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Related Publication(s)
Research paper

highroad Is a Carboxypetidase Induced by Retinoids to Clear Mutant Rhodopsin-1 in Drosophila Retinitis Pigmentosa Models.
Huang et al., 2018, Cell Rep. 22(6): 1384--1391 [FBrf0238009]

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