The work on vine/cct-gamma was carried out at Stanford in the early 2000s in collaboration with Boaz Levi, while I was a postdoc with Mark Krasnow. We generated several deficiencies and I am afraid I do not recall which one we sent out. The best descriptions of this work are from Boaz's thesis:
Df(3R)vine2 was generated by recombination between P{XP}d07129 and P{WH}f01711, Df(3R)vine5 between P{XP}d07727 and P{RB}e04092, Df(3R)vine6 between P{XP}d07727 and P{RB}e02761, and Df(3R)vine7 between P{XP}d07129 and P{RB}e03221.
Regarding the rescue construct:
The pCaSpeR4 Cctγ genomic rescue construct was generated by PCR amplification of genomic DNA with Pfu DNA polymerase in two parts: primers vn79kf (5'-CTTGCTGGGCAGAATGTTTG) and Cctg1R (5'-CAAACTCCTCCTCGATCTGC) to make CctγA; and CG14906.1r (5'-CAGCCTTACGAGATGCTCTAC), and Cctg1F (5'-GGTTCTAACCTCACTCATTCTC) to generate CctγB. These were then subcloned into pCRBlunt-II (Invitrogen). CctγA fragment was isolated with Xho1 and Spe1 digest, while CctγB fragment was isolated with Xho1 and Not1 digest, and then subcloned into the pCaSpeR4 Spe1 and Not1 sites. The construct was verified by sequencing and injected into yw flies.
Best Regards,
Amin
Amin S. Ghabrial
Assistant Professor of Pathology and Cell Biology
Columbia University