FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
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Hu, C. (2019.8.28). DRSC predicted short-guide RNAs, update.  ( Link 1 )
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FBrf0243251
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Personal communication to FlyBase
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The DRSC has generated an updated list of about 11 million predicted short-guide RNAs (sgRNAs) that are suitable for genome engineering using CRISPR/Cas9, using the methods described in Ren et al., 2013 (FBrf0223428). Each sgRNA has been assessed in various ways for predicted efficacy and specificity.
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Related Publication(s)
Research paper

Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9.
Ren et al., 2013, Proc. Natl. Acad. Sci. U.S.A. 110(47): 19012--19017 [FBrf0223428]

Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi.
Housden et al., 2015, Sci. Signal. 8(393): rs9 [FBrf0229582]

Personal communication to FlyBase

DRSC predicted short-guide RNAs.
Hu, 2018.9.24, DRSC predicted short-guide RNAs. [FBrf0240523]

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File date: 2019.8.28 ; File size: 238284865 ; File format: gff ; File name: Hu.2019.8.28.sgRNA_designs.tar.gz
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    English
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