1. A cross between the two stocks:
11632 P{PZ}l(3)77CDf04521 ry506/TM3, ryRK Sb1 Ser1
42044 y1 w*; Mi{MIC}CG4825MI01234/TM3, Sb1 Ser1
resulted in 80 balanced progeny and 4 non-balanced, indicating that the P{PZ}l(3)77CDf04521 insertion line fails to complement Mi{MIC}CG4825MI01234.
2. A cross between the two stocks:
5479 l(3)77CDfY6/TM6C, Sb1 Tb1
42044 y1 w*; Mi{MIC}CG4825MI01234/TM3, Sb1 Ser1
Resulted in 55 balanced and 0 non-balanced progeny, indicating that l(3)77CDfY6 fails to complement Mi{MIC}CG4825MI01234.
3. A cross between the two stocks:
11632 P{PZ}l(3)77CDf04521 ry506/TM3, ryRK Sb1 Ser1
5479 l(3)77CDfY6/TM6C, Sb1 Tb1
resulted in 103 Sb1 progeny and 36 Sb+ progeny. All the Sb+ progeny have a Minute phenotype (shorter/thinner bristles), indicating that l(3)77CDfY6 and P{PZ}l(3)77CDf04521 complement each other in terms of viability (although the resulting transheterozygous flies do have a mutant phenotype).
4: A cross between the two stocks:
5479 l(3)77CDfY6/TM6C, Sb1 Tb1
27368 w1118; Df(3L)BSC796/TM6C, Sb1 cu1
Resulted in 77 Sb1 and 0 Sb+ progeny. Df(3L)BSC796 uncovers CG4825, consistent with l(3)77CDfY6 affecting CG4825.
On balance, given that both l(3)77CDfY6 and P{PZ}l(3)77CDf04521 fail to complement Mi{MIC}CG4825MI01234, this suggests that the l(3)77CDf complementation group maps to the CG4825 annotation.