The following information accompanied stocks donated to the Bloomington Stock Center by Cintia Hongay, Clarkson University, USA.
Mutations were generated in the endogenous Mettl3 (Dm ime4) locus via multi-step CRISPR followed by homologous recombination. To generate each mutant, a GFP cassette was first inserted in place of either an N-terminal or C-terminal portion of the Mettl3 coding sequence. This intermediate sequence cassette was then replaced with the desired sequence using CRISPR/Cas9-mediated homologous recombination.
TI{UAS}Mettl3UAS	Sequence carrying UAS has been inserted just upstream of the ATG of Mettl3. Other sequences in the gene and upstream region of Mettl3 are unchanged.
TI{TI}Mettl3N.HA	Sequence carrying HA has been inserted just after the ATG of Mettl3. Other sequences in the gene and upstream region of Mettl3 are unchanged.
TI{TI}Mettl3N.GFP	Sequence carrying GFP has been inserted just after the ATG of Mettl3. Other sequences in the gene and upstream region of Mettl3 are unchanged.
TI{TI}Mettl3Ala1	The VVMADPPW motif of Mettl3 has been mutated to VVMAAPPA. Other sequences in the gene and upstream region of Mettl3 are unchanged.
TI{TI}Mettl3Ala2	The KIEL motif of Mettl3 has been replaced with Alanines. Other sequences in the gene and upstream region of Mettl3 are unchanged.
TI{TI}Mettl3C.HA	Sequence carrying HA has been inserted at the C-terminus of Mettl3. Other sequences in the gene and upstream region of Mettl3 are unchanged.
TI{TI}Mettl3C.GFP	Sequence carrying GFP has been inserted at the C-terminus of Mettl3. Other sequences in the gene and upstream region of Mettl3 are unchanged.
The Hongay.2019.12.17.pdf file associated with this personal communication contains figures that show the technique used to generate each mutant and their molecular structure.