Akhtar, J., More, P., Albrecht, S., Marini, F., Kaiser, W., Kulkarni, A., Wojnowski, L., Fontaine, J.F., Andrade-Navarro, M.A., Silies, M., Berger, C. (2019). TAF-ChIP: an ultra-low input approach for genome-wide chromatin immunoprecipitation assay.  Life Sci Alliance 2(4): e201900318.

FBrf0244569

Research paper

Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is a powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a tagmentation-assisted fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality histone profiles from low cell numbers. The data obtained from the TAF-ChIP approach are amenable to standard tools for ChIP-Seq analysis, owing to its high signal-to-noise ratio. The epigenetic profiles from TAF-ChIP approach showed high agreement with conventional ChIP-Seq datasets, thereby underlining the utility of this approach.

PMC6653780 (PMC) (EuropePMC)