FB2026_03 , released September 17, 2026
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Citation
Luo, J., Schnitzer, M. (2020.4.23). Act5C-eyDBD.Cas9 constructs and insertions. 
FlyBase ID
FBrf0245562
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Personal communication to FlyBase
Abstract
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Text of Personal Communication 
The following information accompanied stocks donated to the Bloomington Stock Center by Junjie Luo and Mark Schnitzer, Stanford University.
M{Act5C-eyDBD.Cas9} expresses Cas9 protein fused to the ey DNA-binding domain ubiquitously under the control of Act5C regulatory sequences to recruit 3xP3-containing DNA fragments to Cas9-induced breaks and enhance their incorporation upon DNA repair.
M{Act5C-eyDBD.Cas9}ZH-2A is an insertion into the M{3xP3-RFP.attP}ZH-2A attP target site at 2A3,  X:1451892..1451892 .
M{Act5C-eyDBD.Cas9.w-.RFP-}ZH-2A  was derived from M{Act5C-eyDBD.Cas9}ZH-2A using Cre recombinase expressed from P{Crew}DH1 to remove the loxP-flanked miniwhite and Disc\RFP3xP3.PB cassettes.
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    Language of Publication
    English
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    Alleles (2)
    Genes (2)
    Natural transposons (1)
    Insertions (3)
    Experimental Tools (2)
    Transgenic Constructs (2)