Below are explanations of the modifications in the four lines produced for the paper FBrf0245606 Samuels et al., 2020, Biol. Open 9(5):bio049684. 1. proslongSTOP This is a 26 base deletion introducing a stop codon downstream. Below is the final sequence (i.e. without the deleted 26 bases), including 50 bases upstream and downstream of the deletion. CAGTTTCAGCAGCAACAGCAACAGCAACAGCAACAGCAACAGCACCATCAGCAACGGTCACACGTCACCAATACCCTCACAAGTCAACGGCGACGGCAGC 2. proslong-SV40 and proslong-REDr These are two insertions that occur at the same site, inserting an SV40 and a dsRED sequence respectively. Below are the flanking region sequences upstream and downstream of the insertions. Sequence upstream of insertions: ACCAACCAACCAAACGACCAACCTCCGTCAGAAGAATTTTTGGCAATTGTCACCACAAAATTGGAACTGATTGGCAAGCACAACACGACCACCACCACCT Sequence downstream of insertions: TCACACATCTCACTAAACCACAGCCACGAAAACAAGTTCAACTAATCGGTGGAGCGCAGAGGCGAGAAGGGTAAGCGAAATTCATTAAAATTCATCTTAT 3. prosΔprosUP Deleted sequence in prosΔprosUP : GCATGAGTTAGTAGGAATTTATGCTGGCTCTATTATTGTTGCTCTTGTTGTCGCGGATCCTTTTTGGCGGGGTATGAGTGCGGACGACGCTCTACATTTTTTTCAGCTGCAGTTTTCTTTGCTGGATATTTTAATGCCAACTCTGACGATATACCAATACCAGTGCCAATAACCAACACCCATAAATGGCGGCAAAATAAAATAGGAAAAAAATGTGTGTTGCATTCGCGCAGGGGCAACATAGAAAACTTTTACGTTTCGCTTAGGGTTTTGCTTTATTTTTTGCTTTCAGGTTTCCCTCTTCTTTTTTTGCGATTTTCATTTTGTTGCTTTGGCATTGCAAATGAAAACGAAAAAATTGCATTTTCCCCGCATATGGATGTGTGTGAGAAGCACGAAATCAAAACAAAACATGGCCACATGGCGGGTCCTGTCGGCTGGTGGGTGTGCGGATGGGATGTATGGATGCATGGACGGATGGGGCACATGAACAGTGCACAGCCAGGAGCAACAGCTCCCACAGCTGCCACACTACCTCCCTGCAGATGTATCTGTATCTGCGAAATGGTATGGTCCATCTGGCGGGAAAAGGGGCTGGGTTTTAGGTACACTTAGGCTCGATAGCTGCAGTTTTTCGCCACCCAGTCAGCGAAACAGACCACCACGCAGTTGCCCAGTCCAAAAACCACCTCATCAGTCGTTTGGGGGTTTGGGTGTTCCATACTTTTTGGCGTTGTTTGATTTTTAGGCGCAGCAAAATGCGTTTTGCTCTTGATTGAAAGTGTGAAGAGTGTGGGGCAGCATGCCAGCGTGTGTGTGTGCGATATGTGCATTGTCCGTGTCCTTCTCGCAGTGTGCATGTATTGGCGAGGACGCCGCTGCCGCTGTCGTCGCGCTTTGTTTTGATTTTTCGCGACTGAGCGCGCGTTGCTGTCTGTTTCTCTCTGTGTTTTTGGATTTGTCCAGCAATGCATATGTGCGCCCACTGTTCTAGTGGGTGTGTGTGTGTGTTTGTGGTTGTGTGCGAGGTTTGCATGTATATGTACACTAGCAGCTAGGAGCGGCGCTGACGTCGCAGTCGCAGCAATTTTTTGCGCCAAAGCCAAACCAAAAAGGACGAGGACAATTCGGTTGGCGCTGCAGCTGCGACGTAGCCGCGGAATTTTTTTGCGCGTTTCGCGCCCGGCCAGCGGCCACTTTCATCCGTATCCACGTATCCAAGTATCCACATCCGCATCATCCTACTCCATCAGCGTTAGCATCAGCGGCCGAGGGGCTGGCGTCCCTCATAATCAACGCGCTGCAGCGACGACGCCATCGACATCGACAGCGGCTCTGGCTTTGGCTTTGGCTCTGTGACTCTGGGGCCCGTCATCGTTAGCAATTTTTGCCAGGACATTGCAACAGAAAACTGCTAAACGCGAAACAAGTTGCGGCGCTTTTTTCTGCTCCTCGGGTTCTCGCTGTCTCCCTGTCCTGGCCTGTCCTGAATCGAACTGAGCCGTGCTGCCAGGATCAGGCAGCAGCAGGCAGCAGACAGCAGGCAAGGCACATCAGTAGGCAGTGCAAGGCAGGCAGGCAGGCAGTCGGCAGGCAGGTCGCCACTTCTCGCAGACTTCTTCGCATCTGCATAAATCAAACCCAGAGGCAGTAGCAGAAGCAGTTAGCCACCCCAAGAAAAAACTCTACAACAGAAAAATACCGAAAAACAAAAGAAAAACAAACAAACAAATAGAAATAGAAAAGAGAGACATCGAAGCAGAGAAACGATAGGAGAACGAAAAAAAGTGCCAAGTGAATGTGAAAGTGAAAGTGTGTGCAAAATGACTTGAAGTGCAAGACTAGACACCTATGTACGTACCACCTACACCACCCAGCCTGGAACACACTACGTATACGCAATGCGGCCCAGGCGCTCATACGCCCCGTTGGCCCGCCAGCCCTTCGAACTAAGACTCGGGCTGGGGCTCGAACAGCCATCGCTCGCCATGCCGTCTTGAGTGCAAAAAATTACCCGCCAAAAACCCAAAAACACACACGGCCGGCTTGCGCCAAAGACAATGTAAGCGCCGGCACTAGAATGTAGAGCAGTTTTTTCTTCATTTCCCAAAACTAACTAACAAAACCAGCCAGCCAACAACAAATAACAAAATAGACAAAGTTACATTTTTACCAATATCGATAATATTAATCACTTGATAATCAGCGCCAGTCAAACGCAACATTTCGTTCGTAAACAACAACTACAACTAGCCACAACAAAGCAAGTCAAAGCCAAGAAGCAACCAAATAAGCAGGCAATACCAGTCAGTCCGTAACGTCAGCAGCAGAAACCAACGAAAAACCAAAATAATAAAGTAAAACGATTGAGTAACTCCAGTTTCAGTACCGCACCGATTCCGATTTGAGCAAAAATTCAAGCGTCTGAATTTGATTTCGATATCGTCTGGCCCGTTCCGTCTTCAAGTCTCGGCCAAAGGTTCGTCATCGTCCCGACACACTCTCAGTTTCTACAAATTCAGACGTAGATCCTTAGTTAATTCACCTTTTGTATTGTGCATTTCGGCAAGCAAATATTTTGGAAAGGATCCCCAGAGAACAAAAAAAAAAAAAGGCGAAGAAGAGCAGAAGTCAACACTCGAAATCGAAAACTACGAAATTACCTGAAAATTTTTAAACAACCAAAAAGTCTATGAATGCAACAAAAATTACACGCTAAATTTTACAAGTGCAAAATTTTCTTATAATAATTTATCAAAAAAATAAAAATAAATTTACAAAAAAAAAAAAAATTAAAAAAGAAGCATAAAAAAACGGAACTTAGTGAGAAAATTAATCAAAGTTTAGAGAGAACATTAAATGCAAACGGAATATCAAAAATTGTTGTGATGTGATATTTTTTCGATGTACTTTTATTTGAGGGTAAGTAAAGATCACTATCAAAAATTCATTTTTCAACCATTACTCCATTCTTTTCATCAATCATTGACTCATTGGGGTTTATTTATCTTTTCAGAATTATAGAATTTAGATAGAGAAATAAGGGAAATAAATACATTCGAAGCGTATATTTTAAGATCTAGGTAATTTAAAGATGTTCTGATTAAGAAAAGTATCTATTATGGGATGCTTGATCATAAGAAAATGATTAACAGTGAGCATTTAACTACTGATCATTTTTGGAAGAGAACGAGAAGAGAACGACATTTAAGACAAATAGTAAATGGATAATATGAGTGTGAAACATTGAGCAGTATTGATTTTCATTAGAAAAATTAGGAAAAGGTTCATTCTTTAATTATAATGTTATCATCTAAGTTAAAATCAATTCAAAGATATACTCTTTTTAAGGCACAAAATCTATTTCACCAACGAAAGAAAGCAGGGCAGTCAAATTTTAGTAACTTAGCTAAACAGCATGTTTATTTCAAAGCGCCAAAATAATAACCATATTAATTGCTACAAGTAAATATTTAAGAGGAAATGTCTCCTTTGGAGTTCCTTAGTTCAGTGGGTCTGAAGATCGCAGAAGTCTTTCTCCGCGATTGAGCTCGGCACTTCCTCCCAAAGTTCTTCGTGTCCTGTCGTCGGGTGACGGCGTTCAGCTGCAGCAGCAACATGCAACAGCATCGTGTTGCTGGTGTGCACGCGGCTCTCCCCAGCAGCAGCAGGTTTAGTTGTAGTTGTAGACTTTTTGCTTTTCTACATCCTCACACACATACAGGCACACAAACGCACACAGCAACAAACACGAGTGCAAGACGCGAAGTTTTGGAGGCATGCACATAATGTAAACAAGGACATGCGCAATGCACCGCGCTGGCTTTCCCCGCTTCCACAACAGGAGAAGTGCATTGCAGGCAATGCAATTGCCAACTAGAAGATAGCAGCAGCCAAAGGGGCAGTGGGTGGCTGGGCAGGATTTTGGTCGCTTTTGGGGGGGGGGGGGGTGTGGTATTGGCGATGGGGTGTTGCTGGGTGGCTGCTTCATTTACCAACGGCTGCACTAGTAAATAGCAGATGGCAGCGAAGTCGGCGTCGGCATCGCTGTCGACGCCAACTTGCCACAGTTTAGATGGGAAAAAAGGAAAACTCCAAAGAGCCAGAGAGAAAGACAGAGTGAGGGAGAGAGAGCGAGCGAGAGTGGGTGAGAAAGAGAGAGCGAGGGCTGCGCAACGAGAGTGGCGAGTGCAGCCGAAAGGATGCAATGCTGCAAGGCAATTGCACTCCAGGACTCTCACACACACACTTACGCACACAAACGCATCCGCAAGCAGCTAGACTGAGGCGGTTGCTTCTTTTTTGGTTGTGTTGTCTCTTTTCGGCTTATTTTTAGAAGTCCTTTGCCCCCTCGCCGGCGCACATCCCACCCCAGCCCCCTTGGCTGCATTTTATTTACTTGACCAGGCCTCGGAAATTTACATGGCCAGCCGTGGGAGGGGGTTGCAGTATCCACAGCAGTAGCAGCAGCTGTTCCTGGAGCCCCAGCACGTGCAGCAAGCAACTGCCCTTCAGCCGCCCAGCCGCCCAGCCACCCAGCCACCCAGCTGCCTGCGTGATTTGTGCGTTGCCAGGATGCTGATGCTGATGATGATGGCGATGGCGATGATGAGGCATCTCGTAATGATCTGGGGCCGGGAAAAAAGGGAGCAGGAGGCGGTGGTGCAGCTGCATTTCCTTGCCAGCAACGCCGTGCGTGGTTGGTCGGCTCGAAAAAGGGTCGGCGGCACTGATGGGGGTTGGGCTGTGATGGGCTGGCTGGATGGATCGAAGTGTGGGCGCGTAGACATTGCGTAGAGTTGGAGCCAATTTTTTTCTATTTCTTGATGGGCAACCTGGTCCGACCGCCTACATCACCCATTCCACCGCCCCCTTTTTGGACCGCGCTTCATTCGCACATCGAGTGGTCAAAGTACATAGATACCCCATGCCCATGTGTCATGTGTGTGTGTGTGTGCTTTTTGCGCGCGCGAACTTTGGCCAAAGGAAAAAAACTTAAACAGAGATGCTTTTACATACGGAGGGGGGGTTTTTTCGGATGGCTCGCTGCATTTTGCGCGTCTGTAGCTTTTTTTGGCGGCCGTCGATGATGGGGTGCGAAGGGTTGGGGCGCGTGGTGTGTTCCCCGGCCTGGTGGAGGCCATGTACGCGTCGTCGTTTGCTAAAGTCCGTGTCCGTGTCGCGATGACGGAACGCCGAACGAACTGAACGCAACGCAACGCAACGCATCGCATCGCATCTATCGGCATCGGAAGGCATCGGCCGGGCACCGCAGTTGGCATACGGCTTTTGGACGTCTTTTGAGTAGAACATTGCTTTTTTATATTCTCGGCTCGACGCACCGCACCGAGCAAAAGAAGTAACGCCAACGCCGCGTAACGATAGCTGCCGCTCCAGCGAGGAGCCGCAGAGTTGGTCCAAGTCGTCGACGGTTCGGTTCAGTTTCGGACTTGCTTATTTTCTAAAAAATCCAGCAACCAGCAACTAGCACACACACACGCCGTCGAGTGGAGTGGCGTGGAGTGAGGCCGCATTATTATATATAAAATATATATATTGCATATATATATACGGTATAGAATATAGATATATATATATAAATGGGTGCAAAGTGCGCGCGCGTTTGCCGCCCCCAAGTCAACCGCCCATGCCTCTCAATCCGCTGAGGGGCTGAGGTTCAGTTGCAGTTCGAGTGCCGTCAAATACTATTTATATACAATAAATGAAATAAAATATATACAAATATATATAAATATATATATATATATGTCTAAAAATATATGTATCCGGTCGCGAGTATCCTTTCGTGTGCGTTCGTGTGTGTGCGTGTGTGTGTGTGCGTCAGCGGGTGTGTGTTACAATAACAGCTTCGATTGAAAGCAAACAACGAAATTCTAATGCAATCATCTGATTTTTTTGACATATCCAATTGATTATTAAACACTCCGCCGCTGCCCCCATAAAGACCAAAATTTAAAACGCTCCATTAAACCAAAACGTTTTGCTGACATCTTGAACCCAAAGATCCAGCCTCGATTGAAACAGTCCAGTCAACGGCGTCCACTCGTCTGCGTCGTCGTCTGCTGGCATCTAACTTCAGAAAACCAAAATTCATAAGATGCTAGTCCCAAGAGAACTGAATCATCCCATGATCGAGATGCTATTTAACTATTAATATTTGCAACGGGAAACCCGCAAAAAGTCCACTGACTGACCTCTCATGTTTGCACACACTCTGATCCTGGTGCAATCCGACCGATACTGCAAGGCACTGCAACCGTTCGAAAAGCCGCCAGATTTTCATAGCGATCCTCTGGAGCGGCTGTCTCAGCTTTTCCGGCTGGACTTGTCGCACCGTTACGCGTTCCCGGCGACGGCGACGCAGACAACGATGCTAGAGTGGCCAACGAACTGCCCTACAGGACTCACCAGGATCCTTGGGATCAGCCAGGTCATAGCCGTGGACACGAGAGAAAATCTGAACACCGTCATGGATAAATAACATCAGTAAATTGTCCTGTACGTGATCGAAAATCGCACATGTCCCATGCTAAAAACCCCCAATGCACTTGACCATCATAATATCATATATCATTCATATGTAGTTAGTTCATAGTGTGATATTCATTTATATTTTCAATTAGACGCTGAGTATAACCAACATTTGAGATTATGTTATGAATTTGAATCCCCTCAAAACATAACAAACTTGTTGTGTATAAAAACGTTTTATTCTGAAGGACATTAAATTTAGTGTTATAAAATGTCAAAATTAAGGACATTCTTTTAAGCTCAAAATTATTTTATATTGGGTGTGGAAAATTTAAAAATGAAAATATTCTTCCATATTTTCTTAATAGATAGATTCAAGTTAGATATTCATATTCATTTCATAGCTTTAACTATTATTTTCATTTTCTCTCCCGTTCTCAACCGCTTTGTTCCCATAAGCTGAACTACTTGTTAGCTTCATTTGAGTATCTCTCTAGCTTTAGCACGTGTTAGCTCGCTTAAACATAAAAAGACTATATTAAAATGGGGGCAAATGTGTAGCAAGTCGAGTTATTAAATGACACAAGCTTAGAGTCCTTTCCCCAGCGAAAGTACCGACGGCAACTCTGAGTGGTTTCTTGTCAGCAATCTGAAAGTAGGGTTCAGATTTTCGAGTATCTTGTGGAATGCATTTTGAACATTGGGGAATCTTCAGTAGAGGAGAATGTTCTGAGAAAGTCATTTTAGACGTGTGGGAGGGATGCTCTTTTTGCCATTCTCCCCTCACTAACACCGGCCTAACCAGGCTCACACACACATTCACACAACACACCCCCTGCCCATTCTCCATTTTGTTAAGCTGCCCCAACTGGCAGCGACGTCGACGTTGGCAGCGCTGCTTGCCCCTCCATGAACGAGTATTTGAAATGCGCACTAGCTCTAGCTCAAATGGCTTATGTGTCTGTGTGGGCTCCCCTCCGTTGTGGCGTTGTGTGCGTGTGTGGGAGAGTATCCGGCTTCGGGTGGGTGGGCAATTCAGGAAGTCCTGGCAGCGCTGCCGCTGATTTTATTTGCAAAGCCCCACTGTGTGTGTTTTTGCCGTTGCCTTTGGCTTTGCAATGCCTCCCTGGGATATATCCTCCTCCCCCCGGACATTCGCGTATTTTTTCGCGGCTGCGGCGGGCAAACATTTTTTGCTGCCAATACACATACTAGCGCACTCACACCCATACACACACACACACACACACACACTGGCTGGGGCAGAGAGCCGCGGCAACGACAACGCAACGACAACGACACCGTCAGCGGTCGTCCTCGGCCTATTCGCGGCGAAATAATTTTTCGCATTTTTAGTACAAAGCTTCACATCAACGTCGTCGTACAAACAAATTTTTATTTTCGGATTTTTCAATTACGTTTTCATTTATTTTTAAGTATTAAGTTTGCGTGCACTCAAAAAAAAAAAAAAAAACCAAAAACAAAAAGCAACTAAATCAGCAACTAAAACTCGAACAAAATCATAAGGAAATTCATATCCAGAAAACAGTTGATAGATCGTGTGTCCAAACATCGAAATCAAAAGCAAAAAAGCAAAAAGGAAACTCATTCAGACCAAAAAGAATCGAACCACATTTACCGGCTTCCAAAAAACGATTCTCAAGTGTTTTTTCGCCGTATCTCCAAAAGTCTCGACTAAACTCTCAACCGGCAGGAACTCTCGACGATTCTCAGTTTGTTATATGTTTCAACTTGTTTGCCCTCCCACAACACAATATCATCAGTCCATTTTAGTTCTTTAAAATATCAAAAAACGAATAGCGAATGATATTTGAAAAGCAAAGATTACGAACAAAGTCGCGAACAAATTGAAAACAAAAAACAAAAAAACACTAGACGAACAAGGACACAAGTTACAAAATTCAAATGTAAAATCAAAAGTACAAAGCAAAAAGATTGTTGGCAACATTTTTATAAGTATATATACGTATTATTATGATTTGTATATATTTTTTATACGAACTCAAAACTACAGAGTCCAAAACTCAATTTTGATACGCAGAATTGCTCATGTGATGCTCCGAATCCTCCGACGGGTAAGCAAATAGTGTGCGTTATCTTCTCCCGATTAGAAGACGCTGGCGGCAGAGCGAAAGAGTAGGGGCGACAGAAAGAGAGGGAGAGAGAGTGAGAGAGAAGTGAAGAGAAAGGGGCTCTATTCTCGGACTTCTCCTTTCGAAAGTGTCTGAACCGGCAATACGAAGACTGCCAGCCGCAGGCTTCGGGTCTTCGTGCCTTCGTGTCTGATTTTCGGCTGCTTCGTGTCCTGCTGCTTTCTAATTGCGTTCTCTCTCTGCCGGCGTCTGTGTGCGTATGTCTGAGTGTGCCAGTGTGAGTGTGTGCGACTGTGAGTGCATTACATTATCCTGAGGCAGCGGCTACACCTACACCTACGCAGCAGCTTCGGCATCCGACTTCCGATTCCGAATCCGCTCCGATTGCGACTCGGCAAGGACCTCAGCTTGCTGCGGCCGCTGCCTGCCTGCTTCTTCTCTTCTAGTCCTTCTTAAGCTACGCGCGCGTACGGTCGTACCACCCGCCCGCCCGCCGCTCATCCCGCTCATCGTCCCGTTCGTGGCCGCTCGTTTTGGCCATCGGCTCCTCCTTGGAGCGCTTCTTCGTGCAGGTATGCGTGCGATTGTGTGTGTGAGCAGGCGCACACTTACTTGCACTTACTGCGTGTAGGAAATTATCTGCTCCCTCTGTCGCTGCCGGCGTCGCCTAATACATATATAAATAATATATATTATTATACGGGATATACATATATTTAAAAGCGACGACGCTCGTGTATATAGAAAAGGCAGCTCGTTATCGCAGATGCCTGGATAAGGGATGCCAGGGAGTAGGGAGAAGGACCGCCTGCTTGCAAGAAAGTGTAGCACACTTATGTGGGCAGGCGTTGACTGAAGCAGCAGCAATAGCAGCAATAGCAGCGGCGGCAGCTGAGACGGAAACGGAAACGGAGTCCGAATCCTCGACTTCGACTTCGACTGAGGCAGCGCCTTGGTCTTCTGCCACTTTACTTTCGCAGTCTAGAATACAAAATTCAGTACGTTTCCAGTCACGCGCGCTGGCAGAAGTTTTGCTTGCGTATGTCTTCTATCGACGACGTCGACGAACGCCGCCTCGAATTGTGTAAAAATTCCAATCGCTCCACATAAATTATTAAAATAAAATAATAATAAAATATTAACAAAACAAAAAAAAAACCAAAAAAAAAAAAAAGTGTAGCGTGCATTAGCTGTGTGTCTGAGTTGAAGCATCAATTCAAATCAGTTTCAAATCAATCAAATCTAATAAAATATATTTATTTTAAATAAAATAAATAATAAATTTAAATTAAATAAATAAATAAATTGCATTATCACATAAACTAGAAATAACTATTTAAAGCCTAGTTCAGCCAGCTCAATCGTCTCGCCCATCGAACCCAGTCACAGATCCTCCTCAATTAGCTCAGGTGTCGCTGTCTTGTGGTACGTGCAACTTGCGGCGCGTTTATTCGCTGCCTTCTTTCGCGATTAGCCAACCAAAGGGCCACACACACTCTCCACAGAATGTGTAAAAAAAAATCGAAAAAATACTAAACAAAAAAACCATCGAACACACCAACACACACGCAATGGGGCGCCACTTGCTGCTGTTGCTGCGGCATTGGTGGCCACATTGCGCATACGACATGTTAGCCCGCAATTATCCTGCCTGCGCATTGGCAGGCGTCGCGTCTTGGCTTTGATGGGGAAAAACTTGGCAGGAAATTATCTAAAGCTCCCAAGGCAGCCAGAGACTCAGATTCGCCGCAGCCCCGCTCAACTTAGTCAGCAACTCTTCACAGACACTCGCACTCACACACACATACCAACAAGCAGGAGGCTATACTTATACTTATATAGTTGAGATACAGCGAGTCGTGTCTAATTTTATATAAATACTCTCACACAGACGCTGGCACACTCGGAGCCGAATATAAAAGCATTGGGGCTTCGTTTCTTGAAAGACATTTGATCGTCTTGGCTGGCAGCGACTGCAGCGCAGCAAAAGCGGTCAGTTAGGAGGTGGTTTGTCCTTAGTCAGTTGGTCACTCAGCTGGGGAACAAGAACAGCGCAATCAGCATTAGTCAGTAATCAGTTATCAGTTACCAGTTACCAGTTCAAGTCCACACTTTGATGATGGTACGTGCCAGGTTGGGGGATGATGAGAGCCTCTCAGAAGGATGTTTTAAATAAATAACTCGCTAAAACCGTGACAAAGTGAGACGAAATTTTACGACTGTGAAAAAAAAAACACAAATATATCAACTCGGAAAATAAATGTTGTGAATTCAAACAGTGCTACAAATAATACCAATAAATGACTAAATATGAAATAAATCAATTAAACATGTTTTAAAATTAAATAGATATTTGTATTATCTTTATATTTAATTTTAAGTTGTTACTTTCAGTAATGATCTTTACTGTCTCTTAAATTGTTTTATGTCTGTATTATTTTATGATGTTATTTACTGTGTAAAGATTTCTATATGTACTTCTTTAATACCATTTAAAGAAAAAGAAAATTTCTGAGAATAATGGATTTTTATTCTATTATTTTTGTAATCTTCGAACTTAAATTTTAATAAATATATTATATTTCTTCATGAACAGAATATCGAAATTTAGCTAAAAATAAAAACAAAGCCATAAAACAAAACACAAAAAAAGTCTTACAAAATAAATAAGAAAGCAAAAATGATCGAAATTCTAAATTAAACTTGTAAAATCGAAAGCGCTGCGGCAACAAACCTTTGGAAAACCCTTTCGAAAGAAACGAATAAAATAACTAATATATGCGAAATAACTACAACCAAATAACCGGAAATGCTCTGAAACAGTGAAAAGTGTGGCATAGTTTTCGGTGTGCCGCGAAATGAAGTGACAACAAAAGTAGCCAGCCGTGTGGCGGTGCCTTAAAAATAAAATGATAAATGATTCCGTTAGCGACTAAAGAAAAGTTACAAAAATTAGTGACCCCCGATGACCCCACTCCCCACGGCCCCCGTTGACCGCAGCGCTAGAGTGAATTGTGTTCGCCTCTCCCCAAGTCCCAAACGAAATTTACCCCCTGAAAGCAAAAAGTCTGTGCACAGCGACTCGGCCAGAGATATGAGTCGTCCTCATCCGAATTCCGAAATGCATCACCAGCGACCGGAGCGGTCAGCGGCCAACTCCCGCCCGCTCCTGCTCCTGCTCCTGGCAGCGGTACAGTTCCGCTCATCGGGAGCGACTGGCGCTGCCGCCGCTCCTGCCGCCAGCTGCCAACGCTGAACACACGGCCGCCATGAAGCGGTGCGATCCTCTGAAGCGGCTCGATTCGCACTTGCCACTCGCCACTGGCCACTGGCATTGTAACTTAAATTAAGGGTAAAATATCGCAAATGGTATTGGTGCAAAAAAAAAAAAAAAACAAAACAAACCAAACAAACAATGAAAACAAACCAAAAATCTATGAACATTTCATCCCAACTCGAACCGAACATTCAAGAAAAGCTTATAGGTTGCGTTTATACAAATTAGTATAGTAAATTTAGGTAATTGAGGGCGCAGATAAAATATAGAGAAGCTGCAAGTTTTTACATATTTTAAATTCATTATTTATTTATAATTTAAGATAGAAATGGAATGGATTGTGATATATTTTTATAACAAATACTGACATATATGTTTAATGATGTGAATTATTTGTAATAATTCTGAAGCTAAAAGTAAAATATAGATTTTATTTTTTAATATTTGTTATTGTAGCTTTTATTATTTATTTTGTTTTCGCTTGCAGCTTGTGACTTGCAAACCCATTCAATTACTGATAAATAATATGATAAATATATAAAAAAAAAACGCCAAATAGCTCGCACTCCAGTTGAGTTAGCTTTTCGACAGAAAAAGAATTCTTAAGACATAGGGCTTGTGAATACCCCCATTGAAAATTCTTCACAACATTTTCAGCCTACCATCTGAATCAATTGACCAGATTACTAATCTCACATTTACGTTGCGCCCTGTGCCATCGTCTGCTTTTTTACTTGCAGGTTACACCATATCGAGAAGTTATCCAATGAGAAAGCCGCTTAAATTTTGGTAAGTCAACTCCAGTTCATCCTCTTACTTTAAGTCTGGGTCCAGTCTTCGTCTAATGAAACGCCTTTGTGCTCGAGTGGACTTCATTTTTTAATTAGAAAGTCAACACACACACACACACACACACTCGCACGGGGGTGGGGCGAAGTATCCTTAATCGAAAAGTGCTGCCATATGGAGAGGCGCCTTTGTGAGCTGCAGGACTCTGCAACCCCCGCCCGCTTGTTTGCCTTTGTTTATTTGTGTGCTACTTAAGATGAAATGGCAAAGAAAACTGACAGAGATGGTTGTTGGGCTACTAGAACT Flanking region sequence and sequence of inserted attP and loxP sites that replace the deleted sequence in prosΔprosUP : AATTGCAGTGGCTGTCGCTGCACTCGCCCACCAATTTTTCTCCGCTTTTTTGATGCGCTTTCCCAGAACCCCCATGTgtagtgccccaactggggtaacctttgagttctctcagttgggggcgtagataacttcgtataatgtatgctatacgaagttatACAAGGATGAGGGTCGGGGCATCTGAATCCTGCATCTGCCCTGTGTCCGTTTCAATTTCTCCTACCAAGTCAGCTCTTCACGCAGACATTTTCTTTGACTTTCCACCCGC
