Neubacher, S., Dojahn, C.M., Arenz, C. (2011). A rapid assay for miRNA maturation by using unmodified pre-miRNA.  Chembiochem 12(15): 2302--2305.

FBrf0250090

Research paper

Without labeling RNA: A new rapid assay for micro-RNA maturation was developed. The assay depends on the cleavage of unmodified pre-miRNAs and the subsequent amplification of the mature miRNA by rolling circle amplification (see accompanying scheme).