The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Aude Maugarny-Calès, Stephane Pelletier and Yohanns Bellaiche, Institut Curie.
TI{GFP}Osi3nlsGFP-T2A was generated by CRISPR/Cas9. GFP tagged with a nuclear localization signal (nls) followed by a T2A sequence was inserted into the endogenous Osi3 gene at the N-terminus of Osi3, resulting in expression of GFP and Osi3 under the control of endogenous regulatory sequences.
TI{GFP}Osi11nlsGFP-T2A was generated by CRISPR/Cas9. GFP tagged with a nuclear localization signal (nls) followed by a T2A sequence was inserted into the endogenous Osi11 gene at the N-terminus of Osi11, resulting in expression of GFP and Osi11 under the control of endogenous regulatory sequences.
TI{GFP}Osi12nlsGFP-T2A was generated by CRISPR/Cas9. GFP tagged with a nuclear localization signal (nls) followed by a T2A sequence was inserted into the endogenous Osi12 gene at the N-terminus of Osi12, resulting in expression of GFP and Osi12 under the control of endogenous regulatory sequences.
TI{TI}Osi11GFP was generated by CRISPR/Cas9. GFP was inserted at the C-terminus of Osi11 in the endogenous Osi11 locus resulting in expression of GFP-tagged Osi11 under the control of endogenous regulatory sequences.
TI{TI}Osi13GFP was generated by CRISPR/Cas9. GFP was inserted at the C-terminus of Osi13 in the endogenous Osi13 locus resulting in expression of GFP-tagged Osi13 under the control of endogenous regulatory sequences.