The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Norbert Perrimon, Harvard Medical School.
P{UAS-TransTimer.v+} is similar to P{UAS-TransTimer} (FBtp0141313) from He et al 2019 (FBrf0243058), only it was generated in pVALIUM-roe instead of pWALIUM-roe and is therefore marked with a v+ rescue gene instead of a w+ rescue gene. It expresses destabilized sfGFP (sfGFP fused at the C-terminus to the PEST sequence, Tag:PEST (mOdc) followed by the self-cleaving 2A motif followed by TagRFP under the control of UAS. Both sfGFP and TagRFP are tagged at the N-terminus with the nuclear localization signal, Tag:NLS (SV40-largeT). P{UAS-TransTimer.v+} can be used to study transcriptional dynamics.
P{UAS-TransTimer.v+}attP2 attP2 is a homozygous viable and fertile insertion on the third chromosome into attP2 (FBti0040535).