The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Fabian Feiguin, International Centre Genetic Engineering and Biotechnology.
M{UAS-hSOD1.F} – UAS sequences control expression of wildtype human SOD1. The transgene was made in pUASTattB using SOD1 sequences from Genbank CR541742.1. The SOD1 amino acid sequence is: MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQstop
M{UAS-hSOD1.G94A.F} – UAS sequences control expression of human SOD1 carrying amino acid change G94A (associated with ALS). The transgene was made in pUASTattB. The SOD1.G94A amino acid sequence is:
MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDAVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQstop
M{UAS-hSOD1.G94A.DeltaZBD} – UAS sequences control expression of human SOD1 carrying amino acid change G94A and deleted for the zinc binding domain. The transgene was made in pUASTattB. The SOD1.G94A.DeltaZBD amino acid sequence is:
MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSHVGDLGNVTADKDAVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQstop
M{UAS-hSOD1.G94A.DeltaEL} – UAS sequences control expression of human SOD1 carrying amino acid change G94A and deleted for the ‘electrostatic loop’. The transgene was made in pUASTattB. The SOD1.G94A.DeltaEL amino acid sequence is:
MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDAVADVSIEDSVISLSGDHCIIGRTLVVTGNAGSRLACGVIGIAQstop
M{UAS-hSOD1.F}ZH-86Fa is a homozygous viable and fertile insertion on the third chromosome into ZH-86Fa (FBti0099700).
M{UAS-hSOD1.G94A.F}ZH-86Fa is a homozygous viable and fertile insertion on the third chromosome into ZH-86Fa (FBti0099700).
M{UAS-hSOD1.G94A.DeltaZBD}ZH-86Fa is a homozygous viable and fertile insertion on the third chromosome into ZH-86Fa (FBti0099700).
M{UAS-hSOD1.G94A.DeltaEL}ZH-86Fa is a homozygous viable and fertile insertion on the third chromosome into ZH-86Fa (FBti0099700).
