The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Fabian Feiguin, International Centre Genetic Engineering and Biotechnology.
M{UAS-HTT.ex1.Q78} - UAS sequences drive expression of exon 1 of the human Huntingtin (HTT) gene with 78 glutamine repeats. The transgene was made in pUASTattB. The HTT.ex1.Q78 amino acid sequence is:
MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPstop
M{UAS-HTT.ex1.Q78.P31A.P57A} - UAS sequences drive expression of exon 1 of the human Huntingtin (HTT) gene with 78 glutamine repeats and amino acid changes P31A and P57A. The transgene was made in pUASTattB. The HTT.ex1.Q78.P31A.P57A amino acid sequence is:
MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLAQPPPQAQPLLPQPQPPPPPPPPPPGAAVAEEPLHRPstop
M{UAS-HTT.ex1.Q78.DeltaP} - UAS sequences drive expression of exon 1 of the human Huntingtin (HTT) gene with 78 glutamine repeats and deleted for much of the poly-proline and proline-rich sequence. The transgene was made in pUASTattB. The HTT.ex1.Q78.DeltaP amino acid sequence is:
MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPstop
M{UAS-HTT.ex1.Q78}ZH-86Fa is a homozygous viable and fertile insertion on the third chromosome into ZH-86Fa (FBti0099700).
M{UAS-HTT.ex1.Q78.P31A.P57A}ZH-86Fa is a homozygous viable and fertile insertion on the third chromosome into ZH-86Fa (FBti0099700).
M{UAS-HTT.ex1.Q78.DeltaP}ZH-86Fa is a homozygous viable and fertile insertion on the third chromosome into ZH-86Fa (FBti0099700).