FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
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Herz, H.M. (2022.4.25). UASt and UASp H3.3 and His4 constructs and insertions. 
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FBrf0253304
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The following information accompanied stocks donated to the Bloomington Stock Center by Hans-Martin Herz, St. Jude Children's Research Hospital.
In http://flybase.org/reports/FBrf0226097.html, Herz et al. generated PBac{UAS-H3.3.FLAG.HA}VK00018 (http://flybase.org/reports/FBti0163130), which expresses FLAG- and HA-tagged histone 3.3 under UASt control, as well as insertions expressing similarly tagged histone 3.3 with amino acid substitutions, e.g. PBac{UAS-H3.3.K9M.FLAG.HA}VK00018 (http://flybase.org/reports/FBti0163131). Herz and colleagues also generated the following insertions by the same approach encoding tagged histone 3.3 proteins with the named amino acid substitutions:
PBac{UAS-H3.3.K4M.FLAG.HA}VK00018
PBac{UAS-H3.3.K4A.FLAG.HA}VK00018 
PBac{UAS-H3.3.K36M.FLAG.HA}VK00018
PBac{UAS-H3.3.K79M.FLAG.HA}VK00018
They also generated insertions expressing tagged wild type and mutant histone 3.3 under UASp control by changing only the transformation vector:
PBac{UASp-H3.3.FLAG.HA}VK00018
PBac{UASp-H3.3.K4M.FLAG.HA}VK00018 
PBac{UASp-H3.3.K9M.FLAG.HA}VK00018 
In addition, Herz and colleagues generated UASt insertions expressing FLAG- and HA-tagged wild type and K20M mutant Histone 4 by similar methods:
PBac{UAS-His4.FLAG.HA}VK00018 
PBac{UAS-His4.K20M.FLAG.HA}VK00018
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