FB2026_02 , released June 18, 2026
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Reference
Citation
Loehlin, D. (2022.4.13). Location data for deletions in GluClalpha. 
FlyBase ID
FBrf0253307
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Personal communication to FlyBase
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Text of Personal Communication 
Most of this info is in the supplementary information, but I'll make an attempt at presenting this with FlyBase in mind.  An annotated version of the reference genome (.gb format, created in Geneious) is in the associated files.
deletion Df(3R)Acsx1dL (dL):  A CRISPR deletion between guides A2 and C1.  Sequence of scarless excision is in Loehlin et al. 2022 supplement.
deletion Df(3R)Acsx1dR (dR): A CRISPR deletion between guides B4 and D1.  Sequence of scarless excision is in Loehlin et al. 2022 supplement.
deletion GluClαdSpc (dSpc): A CRISPR deletion between guides B4 and C1.  Sequence of scarless excision is in Loehlin et al. 2022 supplement.
deletion Df(3R)Acsx1dLR (dLR): A CRISPR deletion between guides A2 and D1.  Retains 3xP3-dsRed marker.
deletion Df(3R)Acsx1dLR2 (dLR2): A CRISPR deletion between guides A6 and D5. Retains mini-w marker.
Sequences of the guide RNA targets (not including PAM). These should be unique in the reference sequence.
>Acsx1-Guide_A2
CAATAACACATCAATGCCGT
>Acsx1-Guide_B4
TTTAATATGCTACAGCCCTT
>Acsx1-Guide_C1
CAAATCAAGTCAAAGTGTTG
>Acsx1-Guide_D1
TAATGTGCTGTACATCCCTT
>Acsx1-Guide_A6
CACAAACATCATTATTCTGG
>Acsx1-Guide_D5
AGTTCTGAAAACATGCAAGT
DOI
Related Publication(s)
Research paper

A tandem duplication in Drosophila melanogaster shows enhanced expression beyond the gene copy number.
Loehlin et al., 2022, Genetics 220(3): iyab231 [FBrf0252881]

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Associated Files
File date: 2022.4.13 ; File size: 80536 ; File format: gb ; File name: Loehlin.2022.4.13_GluCalpha_deletions.gb
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    English
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    Aberrations (4)
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